利用SRAP标记鉴别美洲黑杨及指纹图谱构建  

Identification and Fingerprinting of Populus deltoides Using SRAP Markers

作　　者：郭娟[1] 樊军锋[1] 梁军[2] 李薇[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨陵712100 [2]中国林业科学研究院森林生态环境与保护研究所,北京100091

出　　处：《西北林学院学报》2014年第2期98-102,共5页Journal of Northwest Forestry University

基　　金：国家"十二五"科技支撑专题(2012BAD19B0801)

摘　　要：利用SRAP分子标记对美洲黑杨1-69及与其有亲缘关系的4个美洲黑杨品种进行了分子鉴别与指纹图谱分析。结果表明：从72对SRAP引物组合中筛选出21对引物组合可以将5个品种完全区分开，21对引物共扩增出287条带，条带清晰，平均每对引物扩增条带为13．7条，多态性条带209条，多态性比率为72．89／6，多态性高；各杨树品种之间的遗传相似系数在0．5481～0．7692之间，平均相似系数为0．6587。并选用扩增出的61条特异性条带构建5个杨树品种的分子指纹图谱。聚类分析结果表明，5个美洲黑杨品种间遗传差异较小，其中陕林3号与北抗1号的遗传差异最大。Identification and molecular fingerprinting analysis of Populus deltoides including P. deltoides. cv. ＇Lux＇（I-69/55） and its 4 affinity varieties were conducted using SRAP makers. Twenty one pairs of primers were screened from 72 primer combinations that could produce polymorphic and high quality bands, and identify 5 varieties completely. Two hundreds and eighty seven bands were amplified and 209 bands were polymorphic from these 21 pairs of primers. Each pair of primers amplified 13.7 bands on aver- age, and the percentage of polymorphic bands was 72.8%. 61 specific bands amplified were used for analy- sis of fingerprinting in the 5 varieties. The similarity coefficients of the five Populus varieties ranged from 0. 548 1 to 0. 769 2 with an average of 0. 658 7. Cluster analysis showed that the genetic differentiation a- mong these 5 P. deltoides varieties was low and P. deltoides, cv. ＂SL-3＂ and P. deltoides, cv. ＂Beikang-1（16- 27/92）＂ showed the highest genetic differentiation among five varieties.

关 键 词：美洲黑杨 SRAP 品种鉴别 指纹图谱 

分 类 号：S792.110.4[农业科学—林木遗传育种]