叶用芥菜小孢子离体培养条件  

In vitro microspore culture of leaf mustard

作　　者：吴慧敏[1] 何勇[1] 邵泱峰[2] 朱祝军[1] 符庆功[1] 

机构地区：[1]浙江农林大学浙江省农产品品质改良技术研究重点实验室,浙江临安311300 [2]浙江省临安市农业技术推广中心,浙江临安311300

出　　处：《浙江农林大学学报》2014年第2期315-321,共7页Journal of Zhejiang A&F University

基　　金：浙江省教育厅资助项目(2273000001)

摘　　要：以5个叶用芥菜Brassica juncea var.foliosa品种为试材,研究了游离小孢子培养过程中基因型、低温预处理、高温热激、更新及加液和秋水仙碱等对小孢子胚状体发生的影响。结果表明:有4个品种获得胚状体,各品种间胚状体产量存在显著差异,‘特选九心芥’胚产量最高;4℃低温预处理24～48 h及33～34℃高温热激处理24～48 h对诱导胚状体发生和发育有促进作用;更新和添加培养基处理也可促进胚状体发生,以质量浓度为170.0 g·L-1的蔗糖起始培养48 h后添加等质量浓度90.0 g·L-1蔗糖培养基处理效果最佳;1～10 mg·L-1秋水仙碱24～48 h处理可提高小孢子培养反应,增加供试材料胚产量。In order to improve the isolated microspore culture system in Brassicajuncea var.foliosa, five differ- ent genotypes of Brassica juncea var. foliosa were used to investigate the contribution of genotype, cool-pre- treatment, heat shock, medium replacement and addition, as well as concentration of colchieine on microspore embryogenesis in vitro. Results showed that embryoids were obtained from four genotypes, and significant dif- ferences （P〈0.05, Duncan＇s MRT） were observed for embryo yield with the highest yield being ＇Texuan-ji- uxin＇ . For all euhivars, 24-48 h of ecol-pretreatment at 4 ℃ and heat shock at 33-34 ℃ improved embryo yields. Also, medium addition and replacement enhanced microspore embryogenesis with the best treatment us- ing a constant volume for a 90.0 g·L-1 sucrose medium supplemented to a 170.0 g·L-1 sucrose concentration medium after 48 h. In addition, treating the freshly isolated microspore with 1-10 mg·L-1 colchicine for 24-48 h greatly improved （P〈0.05, Duncan＇s MRT）embryo yields of the four cuhivars. The present results could be used to obtain haploid efficiently for the plant breeding and basic research in Brassica juncea var. foliosa.

关 键 词：园艺学 叶用芥菜 小孢子培养 胚状体 热激 秋水仙碱 

分 类 号：S637.2[农业科学—蔬菜学]