基于香豆素与CdTe量子点间的荧光共振能量转移检测超痕量Hg^(2+)的研究  

Detection of ultra trace Hg( Ⅱ) based on fluorescence resonance energy transfer between coumarin and CdTe quantum dots

作　　者：张霞[1] 韩韵[1] 连盼盼[1] 丁然[1] 吕鉴泉[1] 

机构地区：[1]湖北师范学院化学与环境工程学院,湖北黄石435002

出　　处：《湖北师范学院学报（自然科学版）》2014年第1期30-36,62,共8页Journal of Hubei Normal University(Natural Science)

基　　金：湖北省自然科学基金(2010CDA061);湖北省教育厅创新团队项目和重点项目(D200922005);污染物分析与资源化技术湖北省重点实验室开放基金(KY2010M03)资助

摘　　要：分别合成了3-巯基丙酸稳定的CdTe@SiO2量子点和双臂香豆素金属离子荧光探针前体(BCP),并将BCP固定于CdTe@SiO2表面上形成Hg2+探针-CdTe@SiO2@BCP,建立了基于Hg2+对CdTe@SiO2@BCP量子点的荧光猝灭定量检测Hg2+的新方法。考察了pH值等因素的影响。在最佳实验条件下,可测定5.0×10-8~1.0×10-4mol·L-1的Hg2+,检出限为6.7×10-9mol·L-1,可应用于实际样品中超痕量Hg2+的测定。CdTe@SiO2 quantum dots and bis -hydroxycoumarin fluorescent probe precursor （BCP） metal ions were respec-tively synthesized.A novel mercury ions fluorescent probe CdTe@SiO2@BCP was formed by the fixing of BCP on the CdTe@SiO2 QDs surface.A sensitive and selective detection of Hg 2＋has been developed based on fluorescence resonance energy transfer （FRET） between coumarin and CdTe quantum dots in CdTe@SiO2@BCP quantum dots.Influence of pH on the fluo-rescence was investigated.Under the optimum conditions, the calibration graph was linear in the range of 5.0×10^ -8- 1.0× 10^-4 mol/L of Hg ^2＋with the detection limit of 6.7 ×10^-9 mol/L.And the method has been successfully applied to the deter-mination of trace concentrations of mercury ions in actual water sample .

关 键 词：CDTE量子点 香豆素 荧光共振能量转移 HG^2+ CdTe@SiO2@BCP 

分 类 号：O657.3[理学—分析化学]