鱼腥草素钠对金黄色葡萄球菌生物被膜的抑制作用  

Inhibitory effect of sodium houttuyfonate on biofilm of Staphylococcus aureus

作　　者：史祺云[1] 程威[1] 金琨琪[1] 施晓晨 杨志强[1] 袁鹏[1] 申凤鸽[1] 张瑾[2] 于录[1] 

机构地区：[1]吉林大学人兽共患病研究教育部重点实验室/人兽共患病研究所,长春130062 [2]吉林大学动物科学学院,长春130062

出　　处：《黑龙江畜牧兽医》2014年第4期14-16,20,207,208,共6页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31172364;31000822;31271951);国家"十二五"重大传染病专项课题(2012ZX10003002);深圳市重点实验室建设扶持计划项目(SW201110026);深圳市药物研发中相关化合物测试与解析高端服务公共技术平台项目(深发改2011-705);深圳市战略新兴产业发展专项资金知识创新计划项目(JCYJ20130401173241464);深圳市基础研究计划项目(JCYJ20130401172016183;YJ20120616142424467);深圳市基础研究实验室提升计划项目(ZDSY20120616141302982)

摘　　要：为了研究鱼腥草素钠对金黄色葡萄球菌（S.aureus）生物被膜的抑制活性，试验采用微量稀释法考察了鱼腥草素钠对S．aureus浮游菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC），用琼脂平板法测定了其对生物被膜的最小抑膜浓度（MBIC）和最小杀膜浓度（MBBC）；通过激光共聚焦显微镜考察了鱼腥草素钠对生物被膜的清除能力；通过Western—blot考察了鱼腥草素钠对S．aureus毒力因子分泌的影响。结果表明：鱼腥草素钠对浮游菌有很好的抑制作用（MIC、MBC：16—64μg／mL），对成熟的生物被膜没有明显的抑制作用（MBIC、MBBC〉1024μg／mL），但其亚抑菌浓度在生物被膜形成早期有显著抑制作用，并呈剂量依赖抑制α-溶血素、肠毒素A和肠毒素B的分泌。说明鱼腥草素钠对金黄色葡萄球菌浮游菌和早期生物被膜有较好抑制活性。To study the inhibitory activity of sodium houttuyfonate on biofilm of Staphylococcus aureus（ S. aureus）, a microdilution method was used measure the minimum inhibitory concentration （M1C） and the minimum bactericidal concentration （MBC） against S. aureus ATCC 25923 and clinical isolates UAMS - 1 in suspension. An agar plate method was used to determine the minimum biofilm inhibitory concentration （MBIC） and minimum biofilm bactericidal concentration （MBBC）. The ability of sodium houttuyfonate which eliminated the biofilm was deter- mined using confocal laser scanning microscopy, and the effect of sodium houttuyfonate on the secretion of S. aureus virulence factors was also determined using Western blot analysis. The results showed that sodium houttuyfonate had inhibitory activity against S. aureus in SUSlSension with MIC and MBC values ranging from 16 to 64 μg/mL, and it had no obvious inhibitory effect on the mature biofilms with MBIC and MBBC values larger than 1 024 μg/mL. The sub - inhibitory concentration of sodium houttuyfonate had an inhibitory effect on the early stages of biofilm for- mation, which could significantly inhibit the secretion of α - haemolysin, enterotoxin A and B in dose - dependent manner. The results indicate that sodium houttuyfonate has good inhibitory activity against S. aureus in suspension and the early stages of biofilm formation.

关 键 词：金黄色葡萄球菌 生物被膜 鱼腥草素钠 毒力因子 

分 类 号：S854.43[农业科学—临床兽医学] S853.75[农业科学—兽医学]