家鸭×番鸭杂种上调表达基因PCMTD1的克隆  

Cloning of a hybrid up-regulated PCMTD1 gene in a crossbreed of domestic duck and Muscovy duck

作　　者：单雪松[1] 陈洋[1] 杨雨江[1] 陈辉[2] 

机构地区：[1]吉林农业大学动物科学技术学院,长春130118 [2]通榆县动物疫病预防控制中心,吉林通榆137200

出　　处：《黑龙江畜牧兽医》2014年第4期26-28,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(30700570)

摘　　要：为了研究动物杂种优势的分子遗传机制,研究以北京鸭、法国番鸭及其正反交F1代杂种为材料,采用mRNA差异显示(DDRT-PCR)方法分析不同试验组鸭肌肉组织蛋白L异天冬氨酸(D-天冬氨酸)O-甲基转移酶1(PCMTD1)基因的表达差异,结合抑制性消减杂交(SSH)方法加以验证。结果表明:PCMTD1基因在杂种中表达上调;与鸭PCMTD1基因3'-UTR 795 bp片段进行比对,与鸡(Gallus gallus)PCMTD1同源性为85%,期望值(E值)为0。说明杂种优势相关基因克隆成功。To investigate the molecular genetic mechanism of animal heterosis, Beijing duck, France muscovy duck and the their mutual hybrids were used as materials to analyze the differential expressions of protein -L - isoaspartate （ D - aspartate） D - methyhransferase domain containing （PCMTD1） genes in duck muscle tissues of different test groups using mRNA differential display （DDRT- PCR）. And the display pattern was verified by the method of suppression subtractive hybridization （SSH）. The results showed that the expression of PCMTD1 gene was up - regu- lated in the hybrids. The comparison results of the 795 bp fragment of duck PCMTD1 gene 3＇ - UTR showed that the duck PCMTD1 gene shared 85 percent homology with that of chicken, and the E - value was 0. The results indicate that the gene associated with heterosis is coloned successfully.

关 键 词：PCMTD1基因 杂种优势 MRNA差异显示 家鸭 番鸭 

分 类 号：S834.81[农业科学—畜牧学] Q785[农业科学—畜牧兽医]