E-64对绵羊卵母细胞成熟及胚胎发育能力的影响  

Effects of E- 64 on sheep oocyte maturation and embryo developmental capability

作　　者：常庆玲[1] 蔡文涛[1] 刘梦鹤 孟娜娜[1] 苏文龙[1] 刘秀丽 田树军[1,3] 

机构地区：[1]河北农业大学动物科技学院,河北保定071000 [2]宣化县江家屯乡人民政府农业综合服务中心,河北张家口075100 [3]河北省牛羊胚胎工程技术研究中心,河北保定071000

出　　处：《黑龙江畜牧兽医》2014年第4期55-58,共4页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家科技支撑计划项目(2011BAD19B02);教育部科学技术研究重点项目(211018);河北省自然科学基金项目(C2012204084)

摘　　要：为了研究E-64对绵羊卵母细胞体外成熟及胚胎发育能力的影响,试验在体外成熟基础液中分别添加0,1,5,10μmol/L的E-64。结果表明:与0μmol/L E-64相比,1μmol/L的E-64能显著提高绵羊卵母细胞囊胚率(P<0.05),5,10μmol/L的E-64能极显著提高其囊胚率(P<0.01),但各浓度E-64对绵羊卵母细胞体外成熟率、卵裂率及囊胚细胞数均无显著影响(P>0.05)。To study the effects of E -64 on in vitro maturation of sheep oocytes and embryo developmental capability, the different concentrations including 0, 1, 5, and 10 μmoL/L of E - 64 were added into the basic fluids of the in vitro maturation medium, respectively. The effects of effects of E - 64 on in vitro maturation of sheep oocytes and embryo developmental capability after in vitro fertilization were studied. The results showed that, compared with 0μmol/L of E - 64, 1 μmol/L of E - 64 could significarltly improve （ P 〈 0.05 ） the rate of blastocyst development from sheep oocytes; 5 and 10 μmol/L of E -64 could highly significantly improve（ P 〈 0.01 ）the rate of blastocyst development, but there were no significant effects （ P 〉 0.05 ） of different concentrations of E - 64 on the rates of in vitro maturation and cleavage rate of sheep oocytes, and the cell numbers in blastocysts.

关 键 词：E-64 绵羊 卵母细胞 体外成熟 体外受精 

分 类 号：S826.3[农业科学—畜牧学] S814.4[农业科学—畜牧兽医]