邻苯二甲酸二丁酯皮肤和经口摄入暴露诱发的小鼠肺损伤  

Lung damage induced by dermal and oral exposure to dibutyl phthalate in Balb/C mice

作　　者：李俐[1] 李金泉[1] 问华肖[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室,湖北武汉430079

出　　处：《环境与健康杂志》2014年第2期95-99,共5页Journal of Environment and Health

基　　金：国家自然科学基金重点项目(51136002);国家科技支撑计划重点项目(2012BAJ02B0301)

摘　　要：目的探讨邻苯二甲酸二丁酯(DBP)暴露诱发的小鼠肺部损伤。方法采用皮肤和经口摄入两种暴露方式染毒,在每一种暴露方式中,各选32只雄性Balb/C小鼠随机分为4组,每组8只。皮肤暴露的DBP剂量分别为0、4、40和400mg/kg,经口摄入(灌胃)的DBP剂量分别为0、0.4、4和40 mg/kg,染毒60 d后,对小鼠肺泡灌洗液中白细胞总数及分类进行计数,采用酶联免疫吸附(ELISA)法测定小鼠血清中免疫球蛋白E(IgE)的含量。同时,采用HE染色法观察小鼠肺部气道的病理学变化。最后,通过动物肺功能分析系统检测小鼠气道高反应性的变化。结果两种染毒方式的小鼠肺泡灌洗液中白细胞数、淋巴细胞、嗜酸性粒细胞和血清中IgE均随DBP染毒剂量的升高呈上升趋势,且与对照组相比,皮肤暴露组中DBP染毒剂量≥40 mg/kg,经口暴露组DBP染毒剂量≥4 mg/kg时,差异有统计学意义(P<0.05,P<0.01)。同时,在两种染毒方式中,随着DBP染毒剂量的升高,小鼠肺部气道出现明显病理学变化,但气道高反应性并无明显的改变。结论高剂量DBP暴露(皮肤≥40 mg/kg,经口≥4 mg/kg)可以提高小鼠肺泡灌洗液中白细胞、淋巴细胞、嗜酸性粒细胞和血清中IgE的水平,并且引起明显的气道病理学变化。Objective To investigate lung damage of Balb/C mice induced by dermal and oral exposure to dibutyl phthalate （DBP）. Methods Dermal and oral exposure were chosen in this study. In each exposure pathway,a total of 32 Balb/C male mice were randomly divided into four groups, eight in each. In dermal exposure groups, the doses of DBP were 0, 4, 40 and 400 mg/kg body weight per day, and in oral exposure groups, the doses of DBP were 0, 0.4, 4 and 40 mg/kg body weight per day. After 60 weeks of treatment, total cells, neutrophils, eosinophils and lymphocytes in bronchoalveolar lavage fluid were counted by using a blood cell analysis system, and the concentrations of immunoglobulin E （IgE） in serum of mice were detected by using ELISA. Pulmonary histopathological examination and airway hyperresponsiveness （AHR） test were also conducted. Results In both of dermal and oral exposure groups, the number of total cells, eosinophils, lymphocytes, the levels of IgE and pathological changes in airways increased with the increase of the concentrations of DBP. However, airway hyperresponsiveness didn＇t show any significant change in this study. Conclusion High concentrations of DBP exposure （dermal ≥ 40 mg/kg body weight per day, ingestion ≥4 mg/kg body weight per day） may increase the number of total cells, lymphocytes, eosinophils in bronchoalveolar lavage fluid and serum IgE levels, and may cause obvious pathological changes in the airways.

关 键 词：邻苯二甲酸二丁酯 皮肤暴露 经口暴露 免疫球蛋白E 过敏性哮喘 

分 类 号：R994.6[医药卫生—毒理学]