蒲公英对Lewis肺癌荷瘤小鼠肿瘤组织干扰素-γ表达的影响  

Influence of dandelion extracts on the expression of IFN-γin the Lewis cancer cells of mice

作　　者：罗江秀 钟超 夏承来[2] 

机构地区：[1]深圳市光明新区人民医院药剂科,广东深圳518106 [2]广州医科大学附属第三医院药学部,广东广州510150

出　　处：《中国医院药学杂志》2014年第7期512-515,共4页Chinese Journal of Hospital Pharmacy

基　　金：广东省自然科学基金项目(编号:S2012040006869);广东省医学科研基金项目(广东省卫生厅A2012273);广州市科技计划项目[穗科信字(2013)166号;2013J4100102]

摘　　要：目的:研究蒲公英对Lewis肺癌荷瘤小鼠肿瘤组织干扰素-γ(IFN-γ)表达的影响。方法:建立肺癌荷瘤小鼠动物模型,处死小鼠,采用Wester blot和RT-PCR研究不同质量浓度(0,31.3,62.5,125,250,500mg·ml-1)的蒲公英以及500mg·ml-1蒲公英在不同时间(0,12,24,48h)对肺癌组织IFN-γ表达的影响。结果:随着蒲公英质量浓度和作用时间的增加,肺癌组织IFN-γ表达降低。结论:蒲公英对Lewis肺癌荷瘤小鼠肿瘤组织IFN-γ表达呈现明显的时效-量效关系,提示蒲公英对Lewis肺癌荷瘤小鼠肿瘤组织的抑制作用和IFN-γ有关。OBJECTIVE Identify the influence of dandelion extracts on the expression of IFN-γ in the Lewis cancer cells of mice METHODS By using RT-PCR and Western blot methods, the effects of different concentration of dandelion extracts （31.3, 62. 5, 125, 250, 500 mg·ml^-1） and different acting times of dandelion extracts （24, 36, 48 h） on the expression of IFN-γ in Lewis cancer cells of mice were observed. RESULTS Along with the increase of dandelion extract concentration, the dandelion extracts could decrease the expression of IFN-γ in Lewis cancer cells of mice in a concentration-dependent manner. CONCLU- SION The extracts from dandelion could inhibit the growth of Lewis cancer cells of mice, which related its effect on the ex- pression of IFN-γ.

关 键 词：蒲公英 干扰素-Γ LEWIS肺癌荷瘤小鼠 肺癌 

分 类 号：R285.5[医药卫生—中药学]