芦丁对STZ诱发大鼠糖尿病心肌病ROCKI蛋白的影响  

The Effects of Rutin on ROCK I in STZ-induced DCM

作　　者：孙波[1] 黄秀英[2] 高海梅[3] 陈园园[1] 高海成[1] 卢泽原 李由[3] 陈利[3] 支晨阳[4] 

机构地区：[1]吉林大学药学院,长春130021 [2]吉林大学第一医院,长春130000 [3]吉林大学第二医院,长春130041 [4]长春中医药大学附属医院肛肠科,长春130000

出　　处：《实验动物科学》2014年第1期22-26,共5页Laboratory Animal Science

基　　金：吉林省科技厅发展计划项目(No.201101067);广东天普生化医药股份有限公司项目(2012099)

摘　　要：目的探讨罗布麻提取物芦丁对链脲佐菌素(STZ)诱发的大鼠糖尿病心肌病(diabetic cardiomyopathy,DCM)ROCK I蛋白的影响。方法用STZ诱发糖尿病心肌病模型,24只雄性Wistar大鼠随机分为正常对照组、DCM组(STZ)、阳性药组(STZ+卡托普利)及芦丁治疗组(STZ+罗布麻提取物芦丁),每组6只。治疗组大鼠给予芦丁灌胃,DCM组和正常对照组大鼠给予0.9%氯化钠注射液灌胃。治疗后行大鼠形态学观测、测定胶原蛋白及ROCK I蛋白表达。结果注射STZ后,大鼠胶原蛋白及ROCK I蛋白的表达较正常对照组增加(P<0.05),罗布麻提取物芦丁灌胃后,其表达相对于模型组降低;HE染色结果显示,给予芦丁后心肌损伤程度减轻。结论罗布麻提取物芦丁可减轻心肌损伤,其机制可能与降低ROCKI蛋白表达有关。Objective To investigate the effects of rutin on ROCK Ⅰ protein in STZ-induced DCM.Method DCM model was induced by STZ.24 male Waster rats were randomly divided into control group （n =6）,STZ group （n =6）,rutin ＋ STZ group （n =6）,Positive drug ＋ STZ group （n =6）.The rats of the treatment groups were given rutin,DCM group and normal group were given 0.9% sodium chloride.The index of rats after intervention of rutin morphology,collagen content and ROCK Ⅰ protein were observed.Result After injection of STZ,collagen and ROCK Ⅰ protein expression increased compared with normal control group （P 〈 0.05）.After rutin treatment,its value reduced as compared with the model group.And HE staining showed that the degree of cardiac injury reduced after rutin treatmenr.Conclusion Rutin may regulate the expression of ROCK Ⅰ and reduce myocardial injury.Specific mechanism needs further study.

关 键 词：链脲佐菌素 ROCKI 芦丁 

分 类 号：Q95-33[生物学—动物学]