聚合Cry1C*和VHb基因玉米抗虫耐渍新种质的创制  

Creating Maize New Germplasms with Borer-Resistant and Waterlogging-Tolerant by Pyramiding Cry1C* and VHb Gene

作　　者：张士龙[1] 贺正华[1] 张祖新[2] 邱法展[2] 黄益勤[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所,武汉430064 [2]华中农业大学植物科学技术学院,武汉430070

出　　处：《玉米科学》2014年第2期46-52,共7页Journal of Maize Sciences

基　　金：转基因生物新品种培育重大专项(2009ZX08003-016B);湖北省农业科技创新中心项目(2007-620-001-03)

摘　　要：分别收集转外源抗虫基因Cry1C*、耐渍基因VHb的HiII、A188玉米材料T1代阳性转化单株花粉,与生产上常用的郑58、248、掖478、昌7-2等30多份自交系杂交和回交,采用"200 mg/L的草铵膦初选-PCR检测-表型鉴定"筛选程序对后代植株进行阳性单株筛选,连续进行3次回交和筛选得到BC3家系,将具有相同遗传背景、不同目标基因的家系杂交,随后选择含2个目标基因的个体与轮回亲本回交和筛选,至BC5后自交纯合获得双抗新系11份。所得到的新系在抗虫、耐渍性状上与轮回亲本有显著差异。The pollens from positive plants of T1 generation of Hi Ⅱ and A188, which were transformed with wa- terlogging tolerance gene ＂VHb＂ and borer-resistant gene ＂Cry1C＊＂ through Agrobaterium-mediated transformation system and particle gun bombardment, respectively, and were collected to pollinate more than 30 important inbred lines such as Zheng58, 248, Ye478, Chang7-2 and so on. And then backcross with these inbred lines as recurrent parents. Subsequently, positive plants were obtained by performing ＂200 mg/L phosphinothricin screening-PCR vali- dation-phenotype identification＂ program. The BC3 families were obtained after backcrossing and screening for 3 times continuously, and then the families with same genetic background but different target genes were crossed to gain those individuals possessing two target genes. Further, the above-mentioned individuals were backcrossed with the corresponding recurrent parents, and then the progenies were screened to get goal plants. Eventually, after continuous backcrossing and screening for 5 times, 11 new double-resistant lines containing ＂Cry1C＊＂ and ＂VHb＂ gene were obtained by inbreeding. There were significant differences between the new lines and the recurrent parent in the traits of borer resistance and waterlogging tolerance.

关 键 词：玉米 抗虫 耐渍 聚合基因 新种质创制 

分 类 号：S513.035.3[农业科学—作物学]