铁、镁及钙对微藻Desmodesmus sp.WC08生长和油脂积累的影响  

Effects of concentrations of iron, calcium and magnesium on the growth and lipid accumulation of microalgal strain Desmodesmus sp. WC08

作　　者：张森[1] 刘平怀[1] 王影[1] 罗宁[1] 张玲[1] 杨勋[1] 

机构地区：[1]海南大学材料与化工学院/热带作物种质资源保护与开发利用教育部重点实验室,海南海口570228

出　　处：《广东农业科学》2014年第4期126-130,共5页Guangdong Agricultural Sciences

基　　金：国家科技支撑计划项目(2011BAD14B01);海南省中药现代化科技专项(ZY201327);海南大学植物学国家级重点学科(071001)

摘　　要：采用单因素试验考察微藻Desmodesmus sp.WC08在4种培养基中的生长和油脂积累的,并以海水BG11为基础培养基,研究铁、镁及钙浓度对该海水微藻生长和油脂积累的影响。结果表明,Desmodesmus sp.WC08在海水BG11中的生物量和油脂产量最高,分别为2.42、0.58 g/L,在F/2中的油脂含量最高,为40.80%。铁、镁及钙浓度试验表明,钙浓度对Desmodesmus sp.WC08生物量具有显著影响,而铁浓度和镁浓度的影响较小。同时,铁浓度和钙浓度对其油脂含量具有显著影响,而镁浓度对其油脂含量影响较小。综合考虑,铁、镁及钙对其油脂产量均具有显著影响。Desmodesmus sp.WC08最适生长和油脂积累的培养基为海水BG11,且其铁、镁及钙浓度分别为2×10-5,0.09、0.05 mmol/L时,最利于Desmodesmus sp.WC08的生长和油脂积累。To investigate the effects of concentration of iron, calcium and magnesium on the growth and lipid accumulation of microalgal strain Desmodesmus sp. WC08, this research has tested the effects of 4 culture medium on the growth and lipid accumulation by single factor experiment. It implied that the optimal biomass （2.42 g/L） and lipid productive （0.58 g/L） were obtained in brine BG11, but the highest oil content （40.80%） was gained in F/2. Iron, magnesium and calcium concentrations tests showed that calcium concentration had the significant effect on the biomass of Desmodesmus sp. WC08, and minor effect was for iron concentration and magnesium concentration. At the same time, iron and calcium concentration had significant effects on the oil content, but magnesium concentration just had smaller influence on the oil content. Given lipid productive, the iron, magnesium and calcium all had significant impact. Brine BGll medium was the optimal culture medium for the growth and oil accumulation of Desmodesmus sp. WC08. And the optimal iron, magnesium and calcium concentrations in brine BG11 culture medium were 2x10 5 0.09 and 0.05 mmoFL, respectively.

关 键 词：Desmodesmus SP 铁 镁 钙 生长 油脂 

分 类 号：Q935[生物学—微生物学]