梯度热应激对大鼠核心体温调节及预后的影响  

The gradient heat stress impact on the convalescent core body temperature and prognosis in heatstroke rats

作　　者：付炜[1] 刘亚楠[2] 耿焱[2] 彭娜[2] 雷玉梅[3] 苏磊[2] 唐忠志[4] 

机构地区：[1]南方医科大学,广州510515 [2]广州军区广州总医院重症医学科,广州510010 [3]广州军区广州总医院附属一五七医院急诊科,广州510515 [4]广州军区武汉总医院急诊科,武汉430070

出　　处：《广东医学》2014年第6期820-823,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81101406);中国博士后科学基金资助项目(编号:2012M512181)

摘　　要：目的构建梯度热应激大鼠模型,探讨不同程度热应激对大鼠核心体温(Tc)调节以及预后的影响。方法雄性SPF级SD大鼠随机分为正常对照组、41℃组、42℃组、43℃组、44℃组。除正常对照组置于室温环境下外,其余各组大鼠于39℃进行热打击,直肠热电偶测量直肠温度,代表Tc。监测各组大鼠Tc的变化,频率为10min/次。41℃、42℃、43℃、44℃组分别达到对应的预设温度41、42、43、44℃时取出大鼠,将大鼠放入环境温度25℃的室温环境下,恢复自由进食、水,并继续监测8 h内Tc变化,观察72 h内大鼠的生存情况。结果随热应激的程度不同,大鼠Tc呈现不同的特征性改变。热应激期间,41℃和42℃组大鼠表现为双相式Tc上升,而43℃和44℃组大鼠表现为三相式Tc上升。脱离热应激后,41℃和42℃组大鼠Tc在1 h内恢复正常水平,而43℃和44℃组均出现低Tc,以44℃组为明显。41℃和42℃组大鼠72 h内未出现死亡,43℃和44℃组出现不同比例的大鼠死亡,生存分析显示,44℃组大鼠预后显著差于43℃组大鼠(P<0.01)。结论不同程度热应激对于大鼠Tc调节特征有显著影响,热应激程度越高,恢复期低Tc程度越深,预后越差。Objective To construct the gradient heat stress rat model and thus to study its influence on core body temperature （ Tc ） during heat stress and prognosis .Methods Male SPF SD rats were randomly divided into normal con-trol group, 41℃group, the 42℃group, 43℃group and 44℃group.Except for normal control group , the rats were ex-posed 39℃heat stress .The rectal thermocouple was used to measure rectal temperature as Tc , which was monitored every 10 min.When the corresponding preset temperatures were reached , the rats in the 41℃, 42℃, 43℃ and 44℃ groups were placed in ambient temperature of 25℃.The Tc was monitored within 8 h, and the survival within 72 h was also recorded.Results With varying degrees of heat stress , the Tc of rats was presented in different patterns .Biphasic rise was observed in 41℃and 42℃groups, while triphasic rise was observed in 43℃and 44℃groups.Out of heat stress, the Tc in 41℃and 42℃groups was returned to normal levels within 1 h, while low Tc was observed in 43℃and 44℃groups, and especially in 44℃group.Deaths were recorded in 43℃and 44℃groups, while significantly worse prognosis was re-vealed in 44℃group according to survival analysis （P〈0.01）.Conclusion Different degrees of heat stress have signi-ficant effect on Tc in rats.The higher degree of heat stress , the lower Tc is presented during the convalescence , suggesting worse prognosis .

关 键 词：热应激 实验动物模型 大鼠 核心体温 

分 类 号：R285.5[医药卫生—中药学]