下垂虫草的重寄生现象及重寄生菌的鉴定  

Hyperparasitism of Ophiocordyceps nutans and Identification of the Mycoparasite

作　　者：曹玉朋[1] 张晓瑶[1] 张磊[1] 李春如[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036

出　　处：《菌物研究》2014年第1期16-21,共6页Journal of Fungal Research

基　　金：国家高技术研究发展计划资助项目(2007AA021506);教育部留学回国人员科研启动资金(2009)

摘　　要：在安徽省岳西县鹞落坪自然保护区,发现了1种寄生于下垂虫草(Ophiocordyceps nutans)的重寄生菌。通过形态特征观察和ITS序列分子生物学分析,将其鉴定为枝多头霉(Polycephalomyces ramosus)。其孢梗束着生于下垂虫草子实体的可孕部,顶部膨大呈球形。本种具有产生单个卵形的A型分生孢子的顶侧生排列的A型瓶梗和能形成分生孢子链的B型瓶梗,并在培养基上的气生菌丝和孢梗束的柄部都观察到梭形、链生的B型分生孢子。GenBank中相关种的ITS序列与拉丁名可能有误。More than four specimens of Ophiocordyceps nutans with synnemata were collected from Yaolu- oping Natural Reserve of Yuexi in Anhui province. Based on morphological characteristics and molecular analysis of the internal transcribed spacer rDNA sequence, the hyperparasite was identified as Poly- cephalomyces ramosus. Synnemata of the hyperparasite, arising from the fertile part of Ophiocordyceps nu- tans, were white, usually unbranched and more or less tomentose. Viscous conidial mass formed a con- tinuous cap at the apex of the synnema, containing acmpleumgenous A-phialides and ovoid A-conidia. Morphological observations on the strain grown in various culture media and the stipe of synnema both re- vealed that this species can produce B-phialides and catenate fusiform B-conidia. Some ITS sequences and their Latin names may be inconsistent in GenBank database.

关 键 词：下垂虫草 枝多头霉 ITS序列 重寄生 

分 类 号：Q949.32[生物学—植物学] Q36