诱导前后地鳖血淋巴抗菌活性的研究  

A Study of Antibacterial Activity of Haemolymph from Eupolyphage by Inducement

作　　者：代春迎[1] 谭竹钧[1] 黎子蔚[1] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006

出　　处：《广东工业大学学报》2014年第1期136-140,共5页Journal of Guangdong University of Technology

基　　金：国家自然科学基金资助项目(30772739);广东工业大学211工程培育项目(20091015)

摘　　要：采用体内注射法,对中华真地鳖(Eupolyphage sinensis Walker)成虫(雌性)活体分别注射大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌,诱导24、48和72 h后取血淋巴,以未经诱导的血淋巴为对照,用滤纸片法分别测定诱导后血淋巴对此3种菌的抑制作用.选择抗菌活性最强的金黄色葡萄球菌诱导48 h的血淋巴,分析加样量、pH、温度和加热时间等因素对血淋巴抗菌活性的影响.结果表明:随着加样量的增大诱导血淋巴的抑菌效果增强;血淋巴抑菌活性最适pH值为5.0;血淋巴的抗菌活性热敏性较强,在80℃和100℃下短时间内失活.Eupolyphage sinensis Walker adults （female） were injected with 5 μL E.coli、Staphylococcu-saureus and Bacillus subtilis respectively via intravitreal injection .The antibacteial activity of haemolymph extracted from induced Eupolyphage for 24, 48 and 72 h respectively to the three bacterial was measured with the filter paper method and compared with the uninduced Eupolyphage as the negative control .The strongest antibacterial activity of haemolymph induced by Staphylococcus aureus for 48h was chosen and then the influence on haemolymph of such factors as the increased sample , pH, temperature and heating time wereanalyzed .The results show that the antibacterial activity of hemolymph enhances with the in-creasing amountof the sample; The optimal pH value is 5.0; the antibacterial activity of hemolymph is sensitive to heat ,and thereis no action at the temperatures of 80 ℃or 100 ℃within a short period .

关 键 词：地鳖 血淋巴 诱导 抗菌 

分 类 号：O629[理学—有机化学]