孕妇外周血胎儿ε血红蛋白基因mRNA的表达及与孕龄的相关性  

Correlation between Fetal Hemoglobin mRNA Abundance in Maternal Peripheral Blood And Gestational Age

作　　者：刘红英[1] 杨利丽[1] 杨和军[1] 刘顺梅[1] 张金宝[1] 

机构地区：[1]潍坊医学院遗传学教研室,山东省潍坊市261053

出　　处：《中国全科医学》2014年第7期792-794,共3页Chinese General Practice

基　　金：山东省人口与计划生育委员会基金项目(200906)

摘　　要：目的 探讨孕妇外周血中胎儿ε血红蛋白基因mRNA的表达水平及其与孕龄的相关性.方法 选择2008年10月—2009年11月在潍坊市人民医院行产前检查的7～16周正常单胎妊娠孕妇30例,常规方法提取全血总RNA,采用逆转录聚合酶链式反应（RT-PCR）法扩增胎儿ε血红蛋白基因片段,琼脂糖凝胶电泳,并扫描成像,用ImageJ软件对电泳条带进行图像分析,测定电泳条带的灰度值,计算孕妇外周血中胎儿ε血红蛋白基因mRNA的浓度,并分析其与孕龄之间的相关性.结果 30例样品RNA质量浓度值为0.98～3.19 g/L,平均（1.87±0.76） g/L;光密度平均比值为1.79,7例阳性,23例阴性.孕妇外周血中胎儿ε血红蛋白基因mRNA浓度为0.537～1.790 μg/ml,中位数为1.241 μg/ml.孕妇外周血中胎儿ε血红蛋白基因mRNA的浓度与孕龄呈负相关（r=-0.750,P=0.026）.结论 孕妇外周血中存在胎儿ε血红蛋白基因mRNA且其含量在7～16周与孕龄呈负相关,利用孕妇外周血中胎儿RNA进行无创性产前诊断具有可行性.Objective To investigate changes in fetal hemoglobin （HbF） mRNA abundance in maternal peripheral blood in association with gestation age.Methods Peripheral blood was collected from 30 normal pregnant women （7-16 weeks）undergoing regular prenatal examinations in Weifang People＇s Hospital between October,2008 and November,2009.Total RNA was extracted from the whole blood First-strand HbF cDNA was synthesized through reverse transcription and subsequently amplified by PCR using primers designed based on the human HbF gene.The PCR products were visualized by gel electrophoresis.The abundance of HbF mRNA was accordingly determined with the ImageJ software and its correlation with gestation age was analyzed by Pearson＇s correlation test.Results The RNA mass concentration is 0.98 ～ 3.19 （1.87 ± 0.76） g/L,the OD value is 1.79.And we detected ε hemoglobin gene mRNA in 30 samples,7 are positive and 23 are negative.And the average concentration of ε hemoglobin gene mRNA in maternal peripheral blood is 1.241 μg/ml,A strong negative correlation （r =-0.75,P ＜0.05） was observed between the amount of HbF mRNA in the maternal blood and gestation age.Conclusion The abundance of fetal hemoglobin mRNA in maternal peripheral blood decreases with gestation age in 7-16 week of pregnancy.

关 键 词：孕妇 胎儿血红蛋白 RNA 信使 孕龄 

分 类 号：R714.12[医药卫生—妇产科学]