103例江苏汉族类风湿关节炎患者HLA-G14bp插入/缺失多态性研究  

HLA-G14bp insertion /deletion polymorphism in 103 patients with rheumatoid arthritis from Han population of Jiangsu

作　　者：杭红蕾[1] 李丽[1,2] 崔燕[1] 何成涛[1] 时彦[2] 徐玉蓉[2] 徐百泉[2] 

机构地区：[1]东南大学医学院检验系,南京210009 [2]东南大学附属中大医院检验科,南京210009

出　　处：《临床检验杂志》2014年第3期181-184,共4页Chinese Journal of Clinical Laboratory Science

基　　金：江苏省科技厅社会发展基金(BS2006542);江苏省卫生厅科学技术资助项目(H200757);国家大学生创新性实验计划资助项目(G2007058)

摘　　要：目的探讨江苏汉族类风湿关节炎(RA)患者HLA-G14bp插入/缺失多态性与疾病易感性及其他临床特征的相关性。方法采用序列特异性引物PCR(PCR-SSP)的方法检测江苏籍103例汉族RA患者及104例无血缘关系健康人的HLA-G14bp插入/缺失多态性,分析相关临床特征与不同基因型的关系。结果 HLA-G14bp插入/缺失多态性在健康人和RA患者均显示Hardy-Weinberg平衡,RA患者与健康人对照组比较,HLA-G14bp+/+、+/-和-/-基因型阳性率差异无统计学意义(P均>0.05);RA患者不同合并症及不同病程病例组间HLA-G14bp插入/缺失多态性差异无统计学意义(P>0.05);HLA-G14bp+/+组类风湿因子(RF)阳性率与-/-组比较,差异有统计学意义(P<0.05)。结论江苏汉族HLA-G14bp插入/缺失多态性与RA疾病易感性无关,但可影响患者RF的形成。Objective To investigate the relationship between HLA-Gt4bp insertion/deletion polymorphism and disease susceptibility or other clinical features in the patients with rheumatoid arthritis （RA） from Han population of Jiangsu. Methods The HLA-G14bp insertion/deletion polymorphisms in 103 patients with RA and 104 healthy controls from Han population of Jiangsu were detected with polymerase chain reaction-sequence specific primers （PCR-SSP） , and the relationship between clinical features and different genotypes was analyzed. Results The HLA-G14bp insertion/deletion polymorphism showed Hardy-Weinberg equilibrium in both healthy controls and the patients with RA. There was no any significant difference of gene frequencies for HLA-G14bp insertion/deletion polymorphism between RA patients and healthy controls （ P 〉 0.05 ）. Moreover, there was no any significant difference of HLA-G14bp insertion/de- letion polymorphism in RA patients with different complications or with different courses of disease. However, the positive rate of rheu- matoid factor （RF） in HLA-G14bp ＋/＋ group was significantly different from that in HLA-G14bp -/- group （P 〈 0.05 ）. Con- clusion The HLA-G14bp insertion/deletion polymorphism in RA patients from Han population of Jiangsu is not correlated with the susceptibility of RA, but may affect the formation of RF.

关 键 词：类风湿关节炎 HLA—G14bp 插入 缺失 多态性 

分 类 号：R446.6[医药卫生—诊断学]