益气解毒方对局灶性脑缺血大鼠线粒体损伤的保护作用  

Protective effect of Yiqi Jiedu Fang on damaged mitochondria in rats with focal cerebral ischemia

作　　者：文丽梅[1,2] 李韶菁[2] 吴传鸿[2] 高健[2,3] 王军玲[2,3] 段飞鹏[2,4] 黄志英[2,5] 李德凤[2] 马超英[1] 

机构地区：[1]西南交通大学生命科学与工程学院四川610031 [2]中国中医科学院中药研究所 [3]河北大学药学院 [4]北京中医药大学中药学院 [5]中国药科大学药学院

出　　处：《北京中医药大学学报》2014年第3期175-179,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(No.30901976,81274133,81303261);国家科技重大专项(重大新药创制)资助项目(No.2012ZX09103201-055)

摘　　要：目的探讨益气解毒方及其各组分对局灶性脑缺血大鼠脑组织线粒体的保护作用。方法将SD雄性大鼠随机分为假手术组、模型组、阳性药物银杏叶提取物(EGb761)对照组(4 mg/kg)和益气解毒方、人参总皂苷、黄连素、栀子苷各高、中、低3个剂量组(25、5、1 mg/kg)。大鼠行中动脉栓塞(MCAO)手术前15 min灌胃给药或给予等量生理盐水,手术后24 h进行神经行为学评分,制备缺血侧脑组织线粒体悬液并对其形态及功能指标进行评价。结果与假手术组相比,模型组神经功能学评分显著升高(P<0.01);与模型组相比,益气解毒方高、中、低剂量组及单个组分高剂量组的神经行为学评分均显著降低(P<0.05或P<0.01),且各给药组线粒体的功能均得到不同水平的改善,均可不同水平的改善损伤线粒体的功能;益气解毒方与单组分相比,对局灶性脑缺血大鼠神经行为学及损伤线粒体功能的改善作用更为明显,且呈一定的浓度依赖关系。结论益气解毒方可发挥较好的线粒体保护作用,可能是其发挥抗脑缺血、治疗缺血性脑中风的重要机制,且相对于3个单一组分(人参总皂苷、黄连素、栀子苷)而言,由3个组分组合而成的益气解毒方对各项指标均发挥着相对较好的药理效应,说明益气解毒方组合用药,与单组分给药相比,可更好的发挥整合治疗作用。Objective To investigate the protective effect of Yiqi Jiedu Fang and its different components on cerebral mitochondria in rats with focal cerebra isehemia. Methods Male SD rats were randomly divided into sham-operation group, model group, control group （with positive drug EGb761,4 mg/kg） , high-dose, mid-dose and low-dose Yiqi Jiedu Fang groups （25 mg/kg, 5 mg/kg and 1 mg/kg）, high-dose, mid-dose and low-dose ginsenosides groups （25 mg/kg, 5 mg/kg and 1 mg/kg）, high-dose, mid-dose and low-dose berberine groups （25 mg/kg, 5 mg/kg and 1 mg/kg） and high-dose, mid-dose and low-dose geniposide groups （25 mg/kg, 5 mg/kg and 1 mg/kg）. The model was established withmiddle cerebral artery occlusion （MCAO）. The neuroethology was scored 24 h after MCAO, and suspension of mitochondria from ischemic brain tissue was prepared for reviewing the morphological and functional indexes. Results Compared with sham-operation group, the scores of neuroethology increased significantly in model group （ P 〈 0.01 ）. Compared with model group, the scores of neuroethology decreased significantly in all Yiqi Jiedu Fang groups and high-dose other component groups （P 〈 0.05 or P 〈 0.01 ）. The function of mitochondria was improved at different levels in all medicated groups. Compared with other component groups, the improvements of neuroethological scores and mitochondrial function were more significant in all Yiqi Jiedu Fang groups showing some dose-dependent manner. Conclusion Yiqi Jiedu Fang can play a role in mitochondrial protection, which may be its an important mechanism in antagonizing cerebral ischemia and treating ischemic stroke. Moreover, compared with its three individual components （ginsenosides, berberine and geniposide ）, the combination formula （Yiqi Jiedu Fang） exerted better pharmacological effects to all indexes. It is suggested that combined medicinal administration will play better integrated therapeutic effects than its single component administration.

关 键 词：益气解毒方 人参总皂苷 黄连素 栀子苷 缺血性脑中风 线粒体 大鼠 

分 类 号：R289.51[医药卫生—方剂学]