香蕉枯萎病菌4号生理小种中CHS基因家族的克隆与蛋白序列分析  

Cloning and Protein Sequence Analysis of CHS Gene Family in Fusarium oxysporum f.sp.cubense Race 4

作　　者：毛超[1,2] 杨腊英[2] 戴青冬[1,2] 黄俊生[2] 

机构地区：[1]海南大学,海口570228 [2]中国热带农业科学院环境与植物保护研究所农业部热带农林有害生物入侵监测与控制重点实验室海南省热带农业有害生物检测与控制重点实验室,海口571101

出　　处：《生物技术通报》2014年第3期79-85,共7页Biotechnology Bulletin

基　　金：国家公益性行业(农业)科研专项(200903049);中央级公益性科研院所基本科研业务费专项(2011HZS1J022);海南大学"211工程"建设项目

摘　　要：为了解香蕉枯萎病菌4号生理小种(FOC4)中的几丁质合酶(Chitin synthase,CHS)基因的特点,从而为进一步研究相关基因功能奠定基础。根据丝状真菌中CHS基因设计简并引物,采用RT-PCR的方法扩增得到FOC4中全长cDNA,测序后对该基因家族编码的蛋白进行分析。结果表明,FOC4共有8个CHS基因,根据蛋白聚类分析表明可其可划分为2个家族,并可根据氨基酸序列特征进一步划分为7个小类,该基因家族成员在不同种镰刀菌中保守程度较高。In order to know the characteristics of chitin synthase genes in Fusarium oxysporum f. sp. cubenes race 4 and lay an information foundation for further study of their functions, the degenerate primers were designed according to the sequence of several filamentous fungi’s CHS genes. The FOC4 CHS genes were amplified by PCR and RT-PCR methods. Their cDNAs were sequenced and protein sequences were analyzed. The results showed eight CHS genes were identified in FOC4. Through phylogenetic analysis, the FOC4 CHS proteins can be classified into two families. They also can be classified into seven classes through characteristics of the amino acid sequences analysis. The FOC4 CHS amino acid sequences showed high identity with other Fusarium species by homology camparison analysis.

关 键 词：FOC4 几丁质合酶 序列分析 

分 类 号：S436.681[农业科学—农业昆虫与害虫防治]