一株黄瓜根际促生菌的筛选、鉴定及其发酵特性  

Selection,Identification and Characteristics of a Cucumber GrowthPromotion Strain of Rhizobacteria

作　　者：葛春辉[1] 孟阿静[1] 马彦茹[1] 杨新华[1] 王新勇[1] 孙九胜[1] 

机构地区：[1]新疆农业科学院土壤肥料与农业节水研究所,乌鲁木齐830091

出　　处：《生物技术通报》2014年第3期94-99,共6页Biotechnology Bulletin

基　　金：新疆国际合作交流项目(20126004);新疆农科院优秀青年基金(xjnky-2012-024);国家自然基金项目(31260501)

摘　　要：Salkowski比色法评价菌株发酵产吲哚乙酸(IAA)的能力;采用平皿、盆栽方法检测菌株的促生能力;对典型菌株生理生化测定及16S rRNA基因序列系统发育分析,初步确定菌株的分类地位;进一步采用正交设计探索不同碳源、氮源对菌株产IAA的影响。结果表明从黄瓜植株根部分离得到菌株18株,其中8株为产IAA菌株。菌株SGM7产IAA能力最强,产量达23.59 mg/L;1%SGM7菌悬液对盆栽黄瓜幼苗有明显促生效果(P<0.05);初步鉴定SGM7为短小芽孢杆菌(Bacillus pumilus);其最适产IAA发酵培养基配方为:蛋白胨1%、玉米粉2%、麸皮0.25%、硫酸铵0.05%、硝酸钾0.05%;其发酵液IAA产量高达35.87 mg/L。Salkowski colorimetric was used to evaluate IAA metabolism of different strains, the use of plates and pot trials were conducted to detect that strains improve plant viability;strain was identified by the method of combination strain’ Physiological -biochemical character and 16S rRNA sequence, and further the influence of different carbon sources and nitrogen source in medium on metabolism ability of IAA was explored. Results showed that eight strains that could excrete IAA were isolated from cucumber roots. Strain SGM7 was the highest in IAA excreting capability（23. 59 mg/L in 48 h of cultivation）. Based on its morphological feature, Some of its physiological and biochemical characteristics and its 16S rRNA sequence analysis, it was identified as Bacillus Pumilus. The optimum fermentation medium contained 1% of protein, 2% of maize powder, 0. 25% of wheat bran, 0. 05% of ammonium sulfate, and 0. 05% of potassium nitrate. Strain SGM7 had the highest in IAA excreting capability（35. 87 mg/L in 24 h of cultivation）.

关 键 词：根际促生菌 筛选鉴定 吲哚乙酸 正交设计 

分 类 号：S642.2[农业科学—蔬菜学]