高效液相色谱法同时测定冷饮中禁限用色素胭脂虫红酸、喹啉黄和碱性蓝26  

Simultaneous Determination of Carminic Acid,Quinoline Yellow and Basic Blue 26 in Ice Cream by High Performance Liquid Chromatography

作　　者：顾正健 黄雅佩[1] 沈阳[1] 曹旭妮[1] 

机构地区：[1]华东理工大学食品系生物反应器工程国家重点实验室,上海200237

出　　处：《化学试剂》2014年第4期325-328,342,共5页Chemical Reagents

基　　金：上海市科委资助项目(12142201300;13142200903;12142200803;11142200703;12nm0502300;11nm0505200);上海市教委资助重点项目(12ZZ048)

摘　　要：在儿童食品中对胭脂虫红酸、喹啉黄和碱性蓝26的添加的监控是十分重要的。建立了棒冰样品中同时检测胭脂虫红酸、喹啉黄和碱性蓝263种红黄蓝色素的高效液相色谱定量分析方法，以乙腈-0．1％甲酸水溶液（pH4．0）为流动相，Hydrosphere．C18色谱柱分离，双波长检测器检测，其检测波长随出峰情况分别先后调整为280、410和595Nm；可以实现棒冰样品中添加的胭脂虫红酸、喹啉黄和碱性蓝26的含量分析。该方法胭脂虫红酸和喹啉黄的线性范围均为0．4—100μg／mL，最低检出浓度均为0．2μg／mL，而碱性蓝26的线性范围为0．3—50μg／mL，最低检出浓度均为0．1μg／mL；方法的回收率为90％-98％，峰面积的相对标准偏差（RSD）为0．5％-3．7％。此外，我们还进一步考察了前处理方法中采用聚酰胺固相萃取法实现棒冰样品的净化和富集，与高效液相色谱法联用可以实现上述3种色素的同时分析检测。A method for simultaneous determination of Car- minic acid, Quinoline yellow and Basic blue 26 in ice cream was established by high performance liquid chromatography （HPLC）. The separation was performed in a YMC Hydro- sphere-C18 （250 mm x4.6 mm i. d. 5 μm） column,acetoni- trile and 0.1% Formic acid water solution （pH 4.0 ） were used as mobile phase. UV detector was used to detect at 280, 410 and 595 nm, respectively. The linear ranges of Carminic acid and Quinoline yellow were 0.4 - 100 μg/mL and their detection limits were 0.2 μg/mL. While the linear range of Basic Blue 26 was 0.3 - 50 μg/mL and the detection limit were 0.1 μg/mL. The recovery ranged from 90% to 98% and RSD was 0. 5% - 3. 7%. When Carminic acid, Quinoline yellow and Basic blue 26 were purified by polyamide SPE col- umn and then concentrated by evaporation, the detection level of 0.14 μg/g in ice cream can be measured.

关 键 词：胭脂虫红酸 喹啉黄 碱性蓝26 高效液相色谱 棒冰 

分 类 号：O657.7[理学—分析化学]