细胞凋亡相关Caspases在猪精子冷冻过程中的表达谱研究  

Expression Prolifes of Apoptotic-related Caspases during Boar Spermatozoa Cryopreservation

作　　者：丁丽[1] 方东辉[1] 何莲[1] 彭文培 张燕[1] 曾长军[1] 

机构地区：[1]四川农业大学动物科技学院,四川雅安625014

出　　处：《四川农业大学学报》2014年第1期68-75,共8页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金项目(30109028)

摘　　要：[目的]探究不同冷冻保护剂冷冻猪精子后,精子中Caspases表达模式.[方法]采用普通PCR和荧光定量PCR技术检测Caspase-1～15在添加不同冷冻保护剂冷冻猪精子后的表达谱.[结果]①甘油和乙二醇可以抑制Caspase-2、-8、-13的表达,而DMSO只能抑制Caspase-2和Caspase-8的表达.②海藻糖和乳糖能有效地抑制Caspase-2、-8、-13、-15的表达,而蔗糖能有效地抑制Caspase-1、-6、-13和Caspase-15的表达.③超氧化物歧化酶和过氧化氢酶可以有效防止Caspase-2、-8和Caspase-13的表达,而谷胱甘肽仅可以有效地抑制Caspase-13的表达.④不添加抗冻剂冷冻对凋亡执行子Caspase-3和Caspase-7的表达没有影响.[结论]冷冻保存主要影响凋亡启动子在猪精子中的表达,添加甘油、蔗糖、乳糖能有效抑制猪精子冷冻解冻造成的凋亡.【Objective】To explore Caspases expression prolifes in boar spermatozoa during cryopreservation with different cryoprotectants(CPAs).【Method】This study firstly investigated all 15 Caspases expressions on boar spermatozoa cryopreservation with different CPAs by RT-PCR and qRT-PCR.【Results】① Glycerol and ethylene glycol can inhibit the expression of Caspase-2,-8, and-13,while DMSO can only inhibit the expression of Caspase-2,-8.② Trehalose and lactose can effectively inhibit the expression of Caspase-2,-8,-13and-15,while sucrose can effectively inhibit the expression of Caspase-1,-6,-13and-15.③ SOD and catalase can effectively prevent Caspase-2,-8,and-13from expression.But glutathione was proved effectively in inhibiting the expression of Caspase-13alone.④ Neither Caspase-3or Caspase-7 was responsible for any CPAs.【Conclusion】The influences of cryopreservation on boar spermatozoa were mainly focus on expression of initiator Caspases.Supplement of glycerol,sucrose and lactose were still reasonable to prevent the boar spermatozoa from apoptosis.

关 键 词：猪精子 冷冻保护剂 Caspases表达模式 

分 类 号：S828[农业科学—畜牧学]