凤尾草总黄酮抑制U251细胞迁移的体外研究  

INHIBITORY EFFECTS OF TOTAL FLAVONOIDS IN PTERIS MULTIFIDA ON U251 CELL MIGRATION IN VITRO

作　　者：刘银凤[1] 张龙[1] 杨大为 李玉斌 张海垠 冯继 

机构地区：[1]承德医学院,河北承德067000 [2]秦皇岛第一医院

出　　处：《承德医学院学报》2014年第2期93-95,共3页Journal of Chengde Medical University

基　　金：河北省医学适应跟踪项目(GL2012082)

摘　　要：目的:观察凤尾草总黄酮对人脑胶质瘤U251细胞体外迁移能力的影响。方法:Transwell迁移实验观察不同浓度凤尾草总黄酮(5、10g/L)对人脑胶质瘤U251细胞迁移能力的影响,Western Blot法检测U251细胞IL-6蛋白的表达水平。结果:不同剂量凤尾草总黄酮可抑制脑胶质瘤U251细胞的体外迁移能力,并下调U251细胞IL-6的表达水平。结论:凤尾草总黄酮抑制人脑胶质瘤U251细胞迁移能力的作用可能与其抑制IL-6蛋白的表达水平有关。Objective：To investigate the effects of total flavonoids in pteris multifida on human brain glioma U251 cell migration.Methods：Transwell migration experiment was used to observe the effects of different dose total flavonoids in pteris mulfifida （5, 10 g/L） on U251 cell migration; Western Blot to detect the expression of IL-6 protein in U251 cells. Results：Different dose total flavonoids in pteris mullifida could inhibit U251 cell migration in vitro, and could down regulate the expression of IL-6 in U251 cell.Conclusions： Downregulation of IL-6 protein expression may be the mechanism that total flavonoids in oteris multifida inhibits U251 cell migrationn.

关 键 词：凤尾草总黄酮 人脑胶质瘤U251细胞 IL-6 迁移 

分 类 号：R917[医药卫生—药物分析学]