烟草环斑病毒磁免疫层析试纸条的研制  

Magnetic immunochromatography assay for detection of Tobacco ringspot virus

作　　者：魏梅生[1] 李桂芬[1] 马洁[1] 鲁洁[1] 张永江[1] 孔君[1] 

机构地区：[1]中国检验检疫科学研究院植物检疫研究所,北京100029

出　　处：《植物检疫》2014年第2期24-27,共4页Plant Quarantine

基　　金：质检公益性行业科研专项(201310071)

摘　　要：烟草环斑病毒是我国检疫性有害生物,为防止该病毒传入国内,需建立快速的诊断和检测方法。将烟草环斑病毒的多克隆抗体吸附到磁性纳米颗粒上,研制出磁免疫层析检测试纸条。该试纸条可特异性检测烟草环斑病毒,检测部分提纯病毒的灵敏度为1μg/mL,检测烟草病汁液可稀释1000倍(重量/体积)。该试纸条和线虫传多面体病毒属的番茄环斑病毒、番茄黑环病毒、南芥菜花叶病毒和葡萄扇叶病毒未出现交叉反应,可用于烟草环斑病毒的现场检测。Tobacco ringspot virus （TRSV） is a quarantine pest for China. In order to prevent the introduction of the virus into China, we need to establish a rapidly diagonstic method for use at the point-of-care or in the field. Magnetic immunochromatography assay was developed by coupling magnetic nanoparticles with polyclonal antibody against TRSV. The sensitivity of the method was 1μg/mL for detecting partial purified TRSV. Extract from TRSV infected Nicotiana tabacum cv. White Burley leaf was detected to a dilution of lff3（W/V）. The immunostrip was specific to TRSV. There was no cross reaction with other nepoviruses such as Tomato ringspot virus, Tomato black ring virus, Arabis mosaic virus and Grapevine fanleaf virus. The immunostrip can be used for the on-site detection of TRSV.

关 键 词：烟草环斑病毒 磁免疫层析 试纸条 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]