6种根结线虫的形态和分子检测技术研究  

Morphological and molecular detection methods for six Meloidogyne species

作　　者：何洁[1] 王江岭[1] 陈先锋[1] 王金成[2] 顾建锋[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012 [2]天津出入境检验检疫局

出　　处：《植物检疫》2014年第2期39-42,共4页Plant Quarantine

基　　金：国家科技支撑计划(2012BAK11B03);国家质检总局科研项目(2013IK279);宁波市科技局项目(2011C10010);国家质检总局项目(2011IK176)

摘　　要：进境种苗中截获的根结属线虫多为2龄幼虫,难以鉴定到种。本文对苹果根结线虫、山茶根结线虫、南方根结线虫、花生根结线虫、北方根结线虫和爪哇根结线虫6种根结线虫的幼虫尾形、头帽、口针基部球、DGO及雄虫头冠等鉴别特征进行比较,并结合mtDNA-PCR-RFLP分析,可准确鉴别上述6种常见根结线虫。It is relatively difficult to distinguish Meloidogyne species intercepted from imported seedlings, because most of Meloidogyne nematodes were second stage juveniles. The article proposed that tail shape, head, stylet basal swellings, DGO and head cap of male as important morphological characters distinguish M. mall, M. camelliae, M. incognita, M. arenaria, M. hapla and M. javanica. And on this basis, combined with mtDNA-PCR-RFLP method, those six Meloidogyne species can be easily distinguished

关 键 词：根结属线虫 形态特征 MTDNA RFLP 

分 类 号：S432.45[农业科学—植物病理学]