环介导等温扩增特异检测香蕉穿孔线虫  

Loop-mediated isothermal amplification for detecting of Burrowing nematode (Radopholus similis)

作　　者：熊玉芬[1] 王旭[1] 葛建军[1] 吴品珊[1] 朱水芳[1] 李芳荣[2] 赵立荣[3] 王宏毅[4] 

机构地区：[1]中国检验检疫科学研究院,北京100029 [2]深圳出入境检验检疫局 [3]广东检验检疫技术中心 [4]厦门出入境检验检疫局

出　　处：《植物检疫》2014年第2期48-51,共4页Plant Quarantine

基　　金：质检公益性科研专项(201010256);质检总局科研专项(2011IK156;2011IK183)

摘　　要：香蕉穿孔线虫是一种重要的植物寄生线虫,通过对香蕉穿孔线虫ITS区域序列分析,利用在线软件Primer Explorer V4设计了环介导等温扩增的内、外引物各1对(2条),环引物1对。通过对反应条件的优化,建立了香蕉穿孔线虫环介导等温扩增体系。实验结果表明该方法准确、特异、简便、快速。Radopholus similis is one important plant parasitic nematode species. A specific loop-mediated isothermal amplification （LAMP） primers group, including Rs-3F3, Rs-3B3,Rs-3FIP, Rs-3BIP, Rs-3LF and Rs-3LB for R. similis were designed individually, through alignment and anylysis of internal transcribed spacer （ITS） and 5.8 S ribosomal DNA regions of Radopholus nematodes, using the online biological software Primer Explorer V4 Here, we present a LAMP detection method for R. similis, through temperature optimization. Our experimental results suggest that LAMP detection was precise, specific, simple and rapid.

关 键 词：环介导等温扩增 香蕉穿孔线虫 检测 

分 类 号：S436.681[农业科学—农业昆虫与害虫防治]