臭氧浓度升高对不同品种小麦根际细菌种群的影响  

The effects of elevated O_3 on rhizosphere bacterial communities of different wheat cultivars

作　　者：晏培[1,2] 王秀娟[1] 孙健[1] 宋宇[3] 张惠文[1] 李新宇[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,森林与土壤生态国家重点实验室,沈阳110164 [2]中国科学院大学,北京100049 [3]辽东学院,辽宁丹东118003

出　　处：《生态学杂志》2014年第4期1015-1020,共6页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(41071202)资助

摘　　要：利用臭氧FACE(Free-Air Ozone Concentration Enrichment)实验平台,研究4个小麦品种"扬麦15"、"扬麦16"、"扬幅麦2号"、"烟农19"根际细菌在拔节期和成熟期对臭氧浓度升高的响应;采用变性梯度凝胶电泳(DGGE)和荧光定量PCR(real-time quantitative PCR)方法,分别对细菌种群结构和数量进行了测定。结果表明:臭氧浓度升高仅显著降低了拔节期"扬幅麦2号"小麦品种根际细菌数量,对成熟期各品种小麦根际细菌数量影响不显著;多样性指数和主成分分析表明,"扬麦15"和"扬幅麦2号"根际细菌多样性和种群结构分别在拔节期和成熟期发生明显变化;臭氧对"扬幅麦2号"品种根际细菌种群的影响较持久,可能会产生累积效应。Based on a Free-Air Ozone Concentration Enrichment facility, we studied the responses of bacterial communities in the rhizosphere of four wheat cultivars-Yangmai 15, Yangmai 16, Yangfumai 2, Yannong 19 （Y15, Y16, Y2, Y19） to elevated O3 at jointing and ripening stages. Denaturing gradient gel electrophoresis （DGGE） and realtime quantitative PCR were conducted to reveal community structure and abundance of rhizosphere soil bacteria. The results showed that the elevation of O3 concentration significantly reduced the rhizosphere bacterial abundance of cultivar Y2 at the jointing stage, but had no significant effects on the rhizosphere bacterial abundance of four wheat cultivars at the ripening stage. Diversity index and principal component analyses indicated that the rhizosphere bacterial diversities and community structures of wheat cultivars Y15 and Y2 were obviously changed respectively at the jointing and ripening stages under the treatment of elevated O3 compared with the control. The results suggested that elevated O3 might have a longer effect on the rhizosphere bacterial community of wheat cultivar Y2 than on the other wheat cultivars, which would cause a cumulative effect.

关 键 词：臭氧增高 小麦品种 PCR—DGGE 荧光定量PCR 

分 类 号：S512.1[农业科学—作物学] S154.3