四株昆虫细胞系对粘虫核型多角体病毒敏感性的测定  

Susceptibility of four continuous- culture cell lines to MsNPV

作　　者：于洪春[1] 王晓云[1] 李国勋[2] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]河北农业大学,保定071001

出　　处：《东北农业大学学报》2000年第4期342-345,共4页Journal of Northeast Agricultural University

基　　金：河北省自然科学基金资助!项目编号39916 9

摘　　要：用粘虫核型多角体病毒 ( Mythimna separata Polyhedrosis Virus,Ms NPV)感染粘虫的NEAU- Ms- 94 7311和 NEAU- Ms- 94 10 15,草地夜蛾的 IPL B- SF- 2 1AE,八字地老虎的 Xc730 E四株昆虫细胞系。试验结果表明 ,异源的 IPL B- SF- 2 1AE和 Xc730 E细胞均不能被 Ms NPV感染 ,同源的 NEAU- Ms- 94 7311和 NEAU- Ms- 94 10 15细胞对 Ms NPV的敏感性有明显差异 ,前者在接种病毒后 10 d感染率平均达 39.51% ,形成多角体数目为 2 6.14PIBs/感染细胞 。This paper MsNPV was used to infect 4 insect cell lines.The result showed that two allogenic cell lines, Xc730E and IPLB-SF-21AE, could not be infected with MsNPV.Susceptibility of homologous cell lines,NEAU-Ms-947311 and NEAU-Ms-941015,to MsNPV was very different. Infection rate of MsNPV and yield of polyhedra inclusion bodies reached 39 51% and 26 14PIBs/cell at 10 day postinfection in NEAU-Ms-947311 cell line,respectively,but infection rate of MsNPV was very little in NEAU-Ms-941015.$$$$

关 键 词：粘虫核型多角体病毒 昆虫细胞系 感染 敏感性 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治] S433.4[农业科学—植物保护]