ω-3鱼油脂肪乳剂对急性肺损伤兔肺组织TXB2、6-keto-PGF1α表达的影响  

Influence of ω-3 Fish Oil Fat Emulsion on the Expression of TXB2 and 6-keto-PGF1α in Lung Tissue of Acute Lung Injury in Rabbits

作　　者：扶志敏[1] 王正 杨林 林少霖 欧竹君 王光锁 孙学峰 李国峰 

机构地区：[1]湖南省郴州市第一人民医院中心医院胸心外科,湖南郴州423000 [2]暨南大学第二临床医学院深圳市人民医院胸外科,广东深圳518020

出　　处：《医学临床研究》2014年第2期274-277,共4页Journal of Clinical Research

摘　　要：【目的】探讨ω-3鱼油脂肪乳剂对内毒素脂多糖（LPS）致兔急性肺损伤（ALI）急性肺损伤兔肺组织中血栓素B2（TXB2）、6酮前列腺素F1α（6-keto-PGF1α）表达的影响。【方法】清洁级新西兰兔24只，随机分为对照组（A组）、LPS致伤组（B组）、ω-3鱼油脂肪乳剂预处理＋LPS致伤组（C组），每组8只，记录0h、0．5h、1h、2h、4h时间点氧和指数（PaO2/FiO2）的改变，4h点处死动物，取肺组织测定TXB2和6-酮-前列腺素F1α（6-keto-PGF1α）含量、观察病理变化，并做病理评分。【结果】注射LPS0．5h、1h后B、C两组0．5h、1h兔肺组织TXB2、6-keto-PGF1α均较A组明显升高（P＜0．05），B、C两组比较有统计学意义（P＜0．05），注射LPS2h、4h后三组比较无统计学意义（P＞0．05）；B组TXB2/6-keto-PGF1α（T/P）明显高于A、C两组（P＜0．05），A组与C组T/P均有增高趋势（0．75VS0．73），但两组比较无显著差异（P＞0．05）；A组、C组肺损伤病理组织评分均低于B组（P＜0．05）。【结论】ω-3鱼油脂肪乳可明显减少LPS致ALI的肺组织TXB2含量，对6-keto-PGF1α最终合成无明显抑制，从而显著下调T/P，对内毒素致兔ALI具有一定保护作用。[Objective]To explore the influence of ω-3 fish oil fat emulsion on the expression of TXB2 and 6-keto-PGF1α in lung tissues of acute lung injury （ALI） in rabbits induced by lipopolysaccharide（LPS） .[Meth-ods] A total of 24 clean-grade New Zealand rabbits were randomly divided into control group（group A） ,LPS injury group（group B） and ω-3 fish oil fat emulsion pretreatment and LPS injury group （group C） with 8 cases in each group .The change of oxygenation index（PaO2/FiO2 ） at 0h ,0 .5h ,1h ,2h and 4h time point was re-corded .The animals were killed at 4h .The lung tissues were collected to detect the levels of TXB 2 and 6-keto-PGF1α .The pathological changes were observed and the pathological scores were obtained .[Results] Com-pared with group A ,TXB2 and 6-keto-PGF1α in lung tissues of group B and group C after injection with LPS for 0 .5h and 1h were obviously increased（ P 〈0 .05） ,and there were significant differences between group B and group C（ P 〈0 .05） .There was no significant difference among three groups after injection with LPS for 2h and 4h（ P 〉0 .05） .TXB2/6-keto-PGF1α（T/P） in group B was obviously higher than that in group A and group C（ P 〈0 .05） .The T/P in group A and group C had an increasing tendency（0 .75 vs .0 .73） ,but there was no significant difference between two groups （ P 〉0 .05） .The histopathological scores of lung injury in group A and group C were lower than those in group B（ P〈0 .05） .[Conclusion] Theω-3 fish oil fat emulsion can obviously reduce the level of TXB2 in lung tissues of LPS-induced ALI ,but has no obvious inhibition on fi-nal synthesis of 6-keto-PGF1α ,thereby significantly reduce T/P ,and has protective effect on endotoxin-in-duced ALI in rabbits .

关 键 词：肺 损伤 脂肪乳剂  静脉注射用 6-酮前列腺素F1Α 兔 

分 类 号：R563[医药卫生—呼吸系统]