MSRE-qPCR法检测少弱精子症患者父源印记基因H19上游区域甲基化水平  

The methylation level detection of the upstream of H19 paternal imprinted gene in olig-asthenospermia patient by MSRE-qPCR

作　　者：严鹏[1] 任丽娟 成振 郭大玮[1] 白丽娟[1] 贾琳娜[1] 

机构地区：[1]山西医科大学法医学院,太原030001 [2]山西大医院,太原030032

出　　处：《中国当代医药》2014年第9期12-14,23,共4页China Modern Medicine

基　　金：教育部高等学校博士学科点专项科研基金(2011 1417110003)

摘　　要：目的建立精子中父源印记基因H19上游区域MSRE-qPCR检测方法,并分析男性少弱精子症患者精子父源印记基因H19上游区域甲基化水平。方法收集20例正常精液样本,同时筛选30例少弱精子症患者精液样本,应用甲基化敏感性限制性内切酶法并结合定量PCR对所有样本父源印记基因H19上游区域甲基化水平进行分析。结果正常精液样本父源印记基因H19上游区域平均甲基化率为(99.8±2.72)%,高于少弱精子症患者精液样本的(82.4±15.30)%,差异有统计学意义(P<0.01)。结论 MSRE-qPCR法可用于父源印记基因H19上游区域甲基化水平的检测,少弱精子症者精液样本父源印记基因H19上游区域甲基化水平显著低于正常人。Objective To establish the methylation level of upstream of H19 paternal imprinted gene in semen of olig- asthenospermia patient and analyse the methylation level detection of the upstream of H19 paternal imprinted gene in olig-asthenospermia patient.Methods The methylation level in the upstream of the H19 paternal imprinted gene of ab- normal（30 olig-asthenospermia samples）and normal（20 normal semen samples）was detected respectively by conducting the methylation sensitive restriction endonucleases-quantitative polymerase chain reaction（MSRE-qPCR,asemiquantita- tire DNA methylation analytical method）.Results The average methylation rate in the upstream of the H19 paternal im- printed gene in normal semen samples was（99.8±2.72）%,higher than that in olig-asthenospermia samples（82.4±15.30）%, with statistical differenee（P〈0.01）.Conclusion The method of MSRE-qPCR can be used in the methylation level detec- tion of upstream of the H19 paternal imprinted gene of semen,and the methylation level of upstream of the H19 pater- nal imprinted gene in olig-asthenospermia patient is much lower than that in normal person.

关 键 词：印记基因H19 MSRE-qPCR 少弱精子症 DNA甲基化 

分 类 号：R698[医药卫生—泌尿科学]