Chelex-100法提取DNA用于差异甲基化基因座检测的研究  

The study of Chelex-100 extract DNA method to differential methylation gene loci

作　　者：成振 严鹏[1] 白丽娟[1] 贾琳娜[1] 郭大玮[1] 

机构地区：[1]山西医科大学法医学院,太原030001

出　　处：《中国当代医药》2014年第10期107-109,共3页China Modern Medicine

基　　金：教育部高等学校博士学科点专项科研基金(20111417110003)

摘　　要：目的通过对Chelex-100提取法提取的DNA用于酶切的研究,为差异甲基化在法医实际工作中的应用性研究提供一种快速提取酶切底物的方法。方法 HhaⅠ酶切基因组DNA,PCR扩增,2%琼脂糖凝胶电泳检测,254 nm紫外灯下观察。结果 Chelex-100提取法提取的杂合子样本DNA加酶组只观察到来自父源的等位基因片段,而未加酶组能够观察到来自父源和母源的等位基因片段。结论 Chelex-100提取法提取的DNA能够作为酶切底物应用于差异甲基化基因座的研究。Objective To provide a method of rapid extraction of enzyme substrate for differential methylation applied research in forensic practice by the study of the Chelex-100 extraction to extract DNA. Methods Genomic DNA was digested by Hha ｜ ,with PCR amplification,detected with 2% agarose gel electrophoresis and observed under ultraviolet lamp,whose wavelength was 254 nm. Results The DNA samples with enzyme extracted by Chelex-lO0 from heterozy- gous observed was only the paternal allele fragments,while the paternal and maternal allele fragments were observed in the control group. Conclusion The DNA extracted with Chelex-100 extraction can be used as enzyme substrate,which is applied to study the differential methylation gene loci.

关 键 词：差异甲基化 聚合酶链反应 Chelex-100 

分 类 号：R446.64[医药卫生—诊断学]