藏药镰形棘豆毒性及化学成分相关性分析  

Subacute toxicity and chemical analysis of Tibetan medicine Oxytropis falcata

作　　者：张晓晶[1] 刘伟霞[2] 魏鹏[2] 格桑罗布[1] 确生[3] 张梁[1] 张庆英[1] 

机构地区：[1]北京大学医学部药学院天然药物学系,北京100191 [2]北京大学医学部公共卫生学院毒理学系,北京100191 [3]青海师范大学民族师范学院化学系,青海西宁810008

出　　处：《中国中药杂志》2014年第7期1157-1162,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(20872006)

摘　　要：将镰形棘豆全草分为地上和地下部分,分别用不同溶剂提取得到地上水溶、地下水溶、地上脂溶、地下脂溶4种提取物。各提取物以最大剂量(以生药量计为50 g·kg-1·d-1)连续灌胃小鼠15 d,各组小鼠外观和行为未见明显异常,但脏器系数、血液生化指标、组织病理切片等分析表明地上和地下脂溶性提取物对小鼠肝脏有轻中度损伤,地上和地下水溶性提取物及地下脂溶性提取物对小鼠肾脏有轻度损伤。化学成分分析表明地上和地下脂溶性提取物含有丰富的黄酮苷元,尤以地上部分含量较高,而多糖和蛋白含量较低;地上和地下水溶性提取物则含有较多多糖和蛋白,黄酮苷元含量较低。Four different extracts of Oxytropis falcata, including the aerial aqueous extract, and the underground aqueous extract, the aerial lipophilic extract, and the underground lipophilic extract were prepared and then administrated orally to mice at the maximum dose （50 g·kg-1·d-1 calculated by raw material） for fifteen days respectively. Compared with the control group,which was administrated of 1.0% tween-80, the treatment groups did not show significant differences in appearance and behavior. However, the organcoefficient, blood biochemical indicator and pathological section results showed that the lipophilic extracts of the aerial and underground parts of O. flacata showed mild injury to the liver of mice, while the aerial and underground aqueous extracts and the underground lipophilic extract showed mild toxicity to the kidney of male mice. Chemical analysis showed that the lipophilic extracts of the aerial and underground parts, especially aerial lipophilic extract, consisted of large amount of flavonoid aglycones with little amount of polysaccharides and proteins, while the aqueous extracts contained much polysaccharides and proteins with almost no flavonoid aglycones detected. keywords：

关 键 词：藏药 镰形棘豆 亚急性毒性 化学成分 

分 类 号：R284[医药卫生—中药学]