基于选择性剔除的红花抗氧化效应物质基础研究  

Study on material base of Carthamus tinctorius with antioxidant effect based on selective knock-out

作　　者：王林艳[1] 唐于平[1] 刘欣[1] 葛雅辉 李淑娇[1] 尚尔鑫[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心,江苏南京210023

出　　处：《中国中药杂志》2014年第7期1285-1289,共5页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2008BAI51B01)

摘　　要：目的:建立一种从整体角度研究中药效应物质的方法。方法:以红花为例,利用制备液相色谱剔除其主要成分,然后分别对剔除后的样品进行抗氧化效应评价,进而比较分析得出红花抗氧化活性的主要效应物质及各成分的贡献度。结果:从红花样品中分别剔除了7个主要物质,并通过质谱数据分析和对照品对照鉴定了其中6个成分。通过对包括剔除物质在内的所有样品进行抗氧化效应比较评价,结果发现羟基红花黄色素A,脱水红花黄色素B,6-羟基山柰酚-3,6-二氧葡萄糖-7-氧葡萄醛酸苷是红花抗氧化活性的主要效应物质。结论:该研究探索了一种较新颖而有效的中药效应物质的研究方法,通过这种方法,能从一定程度上阐明不同成分对中药效应表达直接或间接的贡献以及贡献度,使得中药效应物质表达更加明晰。Objective： To establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective. Method： Carthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards, the samples with major components depleted were evaluated for their antioxidant effect, so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions. Result： Seven major components were depleted from C. tinctorius samples, and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect, the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3, 6-di-O-glucoside-7-O-glucuronide were the major efficacious materials. Conclusion： This study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method, we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.

关 键 词：选择性剔除 红花 抗氧化 效应物质 

分 类 号：R284[医药卫生—中药学]