土茯苓总黄酮提取工艺的正交设计优化  

Extraction Process Optimization of Total Flavone from Smilax glabra Roxb by Orthogonal Design

作　　者：陈文龙[1] 陈燕芬[1] 卢传坚[1] 胡梦梅[1] 卢传礼[1] 朱伟[1] 

机构地区：[1]广州中医药大学第二临床医学院,广东广州510006

出　　处：《时珍国医国药》2014年第3期544-546,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81273964);广东省省部产学研结合项目(NO.2011A091000020);南药可持续利用研究与综合开发利用技术平台专项资金资助

摘　　要：目的优化土茯苓中总黄酮提取的最佳工艺条件。方法采用正交实验设计法,以总黄酮得率为指标,考察乙醇浓度、料液比、提取时间和提取温度4个提取因素的最佳水平。结果土茯苓总黄酮的最佳提取工艺为乙醇浓度为50%、料液比为1∶40(g:ml)、提取温度为55℃、提取时间为30 min,总黄酮得率为9.66%。结论该提取工艺简单可靠,合理可行,为土茯苓资源的充分利用提供了科学依据。Objective To optimize the extraction process of total flavonoids from the rhizome of Smilax glabra by orthogonal de- sign. Methods An orthogonal experimental design was employed, with total flavonoids extract ratio as an index, to optimize four extraction factors ： ethanol concentration, solid - liquid ratio, extraction time and temperature. Results The optimum process con- ditions were ethanol concentration 50% （v/v） , solid - liquid ratio of 1：40 （g： ml） , extraction time of 30 min and extraction temperature of of 55℃, and extraction ratio of total flavonoids from Smilax glabra was 9.66%. Conclusion The extraction process is simple and reliable, reasonable and practicable, and it provides the experimental basis for full use of Smilax glabra re- Sources.

关 键 词：土茯苓 总黄酮 正交实验 提取工艺 

分 类 号：R284[医药卫生—中药学]