脾虚大鼠血清及胃组织中胃肠激素SS、GAS和CCK的变化及中药干预研究  

The Research of Variation of Gastrointestinal Hormone,SS,GAS and CCK Content in serum and Gastric Tissue and Traditional Chinese Medicine Intervention of Spleen Deficiency Syndrome Rats

作　　者：孙晓芬[1] 程卫东[1] 段永强[2] 邢文婷[1] 周燚炎 

机构地区：[1]兰州大学基础医学院中西医结合研究所,甘肃兰州730000 [2]甘肃中医学院,甘肃兰州730020

出　　处：《时珍国医国药》2014年第3期756-758,共3页Lishizhen Medicine and Materia Medica Research

基　　金：甘肃省自然科学基金(No.1010RJZA148);甘肃省教育厅基金项目资助(No.0706B-02)

摘　　要：目的探讨脾虚大鼠血清及胃组织中生长抑素(SS)、胃泌素(GAS)和胆囊收缩素(CCK)的含量变化与脾虚证发生的关系。方法将SD大鼠随机分为空白对照组、脾虚模型组、四君子汤组、红芪多糖组。除正常对照组灌服蒸馏水外,其余各组连续制造脾虚模型14d,第15天空白对照组及脾虚模型组采血及取出胃组织制备标本-20℃保存。四君子汤及红芪多糖组分别给予四君子汤及红芪多糖灌胃,继续7d,第22天处理同空白组及模型组。标本均用酶联免疫分析法(ELISA)测定血清及胃组织中SS、GAS及CCK的含量。结果脾虚模型组大鼠血清中SS及CCK比空白对照组显著降低,胃组织中SS及CCK显著升高,GAS在血清及胃组织中均比空白对照组显著降低,差异均具有显著统计学意义(P<0.01);而四君子汤及红芪多糖能使脾虚大鼠血清中SS及CCK显著升高,胃组织中SS及CCK含量显著降低,血清及胃组织中GAS显著升高,差异具有显著统计学意义(P<0.01)。结论 SS、GAS及CCK的分泌水平紊乱是导致大鼠脾虚证发生的主要原因之一。Objective To discuss the relationship between the spleen deficiency syndrome and the content change of somatostatin （SS） , gastrin （Gas） and cholecystokinin （CCK） in serum and gastric tissue. Methods SD rats were randomly divided into nor- mal control group, spleen deficiency model group, Sijunzi group, radix hedysari polysaccharide group, except that the normal con- trol group were given distilled water , the rest groups were manufactured spleen deficiency model 21 days continuously. And the 22th clays normal control group and spleen deficiency model group were drawn blood and were taken stomaeh tissue to prepare specimen - and save them into -20℃. Sijunzi decoction group and radix hedysari polysaccharide group were respectively given intragastric infusion of Sijunzi decoction and intragastric infusion of radix hedysari polysaccharide for 7 days continuously, the 28th days, deal with these two groups same as normal control groups and spleen defieience model groups. Using euzyme linked immu- nosorbent assay（ELISA） method to deficiency the content of SS,Gas and CCK in the serum and the gastric tissue of specimens. Results The content of SS and CCK were significantly lower （ P 〈 0. 01 ） in the serum but higher in the gastric tissue of the group of spleen deficiency rats than normal control group , the content of Gas were significantly lower than normal control group （ P 〈 0.01 ） ; While Sijunzi decoction and radix hedysari polysaccharide can make the content of SS and CCK significantly reduced in the gastric tissue , but increased in the serum , and make the content of Gas significantly increased in the serum and gastric tissue of spleen deficiency rats, this has statistically significant （P 〈 0. 01 ） comparing with spleen deficiency model group. Conclusion The secretion level disorder of SS , Gas and CCK is one of the main reasons to lead to the occurrence of rat spleen deficiency syn- drome.

关 键 词：脾虚证 生长抑素 胃泌素 胆囊收缩素 

分 类 号：R285.5[医药卫生—中药学]