肿节风对小型猪放射性肺损伤的防护作用研究  

Effect of Sarcandrae on radiation pneumonnopathy in miniature pigs

作　　者：张雪燕[1] 刘美莲[1] 蒋健[2] 

机构地区：[1]桂林医学院附属医院放疗科,541001 [2]广西医科大学第一附属医院放疗科

出　　处：《中华放射医学与防护杂志》2014年第3期180-184,共5页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的 探讨肿节风对60Co γ射线照射后的小型猪放射性肺损伤的影响及其机制.方法 将60只小型猪随机数字表法分为3组,每组20只,空白对照组,不加任何处理;单纯照射组,60Co γ射线照射15 Gy;肿节风干预组,于15 Gy照射前服用肿节风配方颗粒（0.3 g/kg）.于照后4、8、12和24周,观察小型猪的呼吸频率和体重,取右肺组织行HE、Masson染色检查,用免疫组织化学法检测肺组织TGF-β1和TNF-α的表达,并检测其羟脯氨酸（HP）含量的变化.结果 照后4、8、12和24周,单纯照射组的呼吸频率、肺系数、羟脯氨酸含量随时间的延长呈递增趋势,均明显高于其他两组（F=21.035、46.014、32.610,P＜0.05）.照后4和8周,肿节风干预组与空白对照组的呼吸频率、肺系数、羟脯氨酸相近（F=0.055、2.456、5.581,P＞0.05）,而至12和24周,肿节风干预组各项指标明显低于单纯照射组（F =91.897、93.149、83.487,P＜0.05）.肺组织病理学观察显示,在照后4和8周,肿节风干预组肺组织无明显炎症反应及纤维化表现,在12和24周纤维化程度明显低于单纯照射组.肿节风干预组在第4和第8周肺组织中的TGF-β1和TNF-α为阴性,在12和24周为低表达,均明显弱于单纯照射组.结论 肿节风配方颗粒对小型猪放射性肺损伤具有一定保护作用,其机制可能是肿节风对TNF-α和HP的表达抑制有关.Objective To explore the effect and underlying mechanism of Sarcandra glabra Nakai on radiation pneumonnopathy in miniature pigs irradiated with 6QCo γ-rays. Methods Totally 60 miniature pigs were randomly divided into blank control group, irradiated group with 15 Gy of 60Co γ-rays, and intervention group administered with Sarcandra glabra Nakai at the dosage of 0.3 g/kg before 15 Gy irradiation. At 4, 8, 12 and 24 weeks post-irradiation, the respiratory rate and weight were observed, and a portion of the right lung tissue was taken out to conduct HE and Masson staining examination, while the expressions of TGF-~I and TNF-a as well as the content of hydroxyproline （HP） were detected. Results The respiratory rate, the lung coefficient and the content of HP in the irradiated group showed an increasing trend at 4, 8, 12 and 24 weeks post-irradiation and significantly higher than those in other two groups （ F = 21. 035, 146. 014, 32. 610, P 〈 0. 05）. Those indicators were indistinguishable between the control group and intervention group at 4 and 8 weeks post-irradiation （ F = 0. 055,2. 456, 5. 581, P 〉 0.05） , while the indieators of the irradiation group were significantly higher than that of intervention group （ F = 91. 897, 93. 149, 83. 487, P 〈0.05） at 12 and 24 weeks post-irradiation. The pulmonary histopathological results showed that no inflammatory response or fibrosis was found in the eontrol group and intervention TGF-I^t and TNF-ct in the intervention group were negatively expressed at 4 and 8 weeks post-irradiation and had low expressions at 12 and 24 weeks post-irradiation whieh was lower than that of the irradiated group. Conclusions Sareandrae has protective effeet against radiation-induced lung injury in miniature pigs probably by inhibiting the expressions of TNF-α and HP.

关 键 词：肿节风 小型猪 放射性肺损伤 TGF-Β1 TNF-Α 羟脯氨酸 

分 类 号：R285.5[医药卫生—中药学]