柔嫩艾美耳球虫第二代裂殖子表面抗原基因(λMZ5-7)的克隆及序列分析  

Sequence Analysis of λMZ5-7 Gene from Second Generation of Merozoites of Eimeria tenella

作　　者：韩红玉[1] 黄兵[1] 

机构地区：[1]中国农业科学院上海家畜寄生虫病研究所农业部动物寄生虫学重点实验室,上海200232

出　　处：《中国预防兽医学报》2000年第5期358-360,共3页Chinese Journal of Preventive Veterinary Medicine

摘　　要：本文选择E .tenella第 2代裂殖子表面抗原基因 (λMZ5_7)为侯选抗原基因 ,利用RT_PCR方法从E .tenella第 2代裂殖子中扩增出了λMZ5_7基因 ,把这一片段克隆到PGEM_T_easy克隆载体上 ,得到的阳性克隆经PCR鉴定及酶切分析。结果表明 ,重组子 (PGEM_T_λMZ)中含有λMZ5_7基因 ,且是正向插入的。核苷酸序列分析结果表明 ,该序列全长 984bp ,有一个开放阅读框 (933bp) ,与国外报道的λMZ5_7基因比较 ,同源性为 98.8% ,只是在 335～ 347bp处缺少 12bp ,其余部分相同 ,缺少的 12bp没有造成氨基酸编码的错位 ,缺少的 4个氨基酸为Thr、Ser、Gln、Gln。A surface antigen λMZ5_7 gene of Eimeria tenella merozoites was cloned by PCR from the synthesized first strand cDNA.The PCR products were analysed by agarose electrophoresis and then cloned into pGEM_T_easy vector successfully.The specific recombinant plasmid was identified with restriction endonuclease analysis and sequenced by automatic DNA sequencer.The results indicated λMZ5_7 gene was 984 in length and included an open reading frame encodes a protein of 311 amino acid residues.Comparison of λMZ5_7 gene we gained with the previously reported λMZ5_7 gene indicated two genes had a high homology of 98.8% and λMZ5_7 gene we gained deleted 12 nucleotides compared with the reported λMZ5_7 gene,but the deleted 12 nucleotides didn't result in change of open reading frame.

关 键 词：柔嫩艾美球虫 第二代裂殖子 序列分析 表面抗原基因 

分 类 号：S852.723[农业科学—基础兽医学]