2009～2011年河北省卢龙地区G9P[8]型人A组轮状病毒全基因组序列分析  

Whole Genome Analysis of Human Group A Rotavirus G9P[8] Strains in Hebei Lulong Region,2009-2011

作　　者：马鑫[1] 李丹地[2] 郭延青[2] 向静瑶[3] 李秀萍[4] 段招军[2] 

机构地区：[1]宁夏医科大学检验学院,银川750004 [2]中国疾病预防控制中心病毒病预防控制所,北京102206 [3]兰州大学第一临床医学院儿科,甘肃730000 [4]宁夏医科大学总医院,银川750004

出　　处：《病毒学报》2014年第2期119-127,共9页Chinese Journal of Virology

摘　　要：了解2009-2011年河北省卢龙地区婴幼儿腹泻样本中A组轮状病毒（GroupArotavirus，GARV）G9PL8]型毒株的基因特征。随机选取2009年至2011年5株G9P[8]型卢龙GARV，采用反转录一聚合酶链反应（RT-PCR）的方法对GARV进行11个片段基因扩增，并对全基因序列通过DNAStar、MEGA等生物软件进行比对、同源性分析及系统进化分析。其中2011年的2株标本LL11131077和LL11131083之间的同源性明显高于它们与2009年和2010年3个毒株的同源性。2009~2011年河北卢龙地区流行的G9P[-8]型GARV与世界其他地区流行的G9P[8]型GARV毒株均属同一基因型，但是2011年的2个毒株与2009年和2010年相比部分基因片段系统进化树不在一个亚枝内，同时也发生了多位点的氨基酸变异。This study aims to investigate the genetic characteristics of group A rotavirus （GARV） G9P[8] strains from infantile diarrhea samples in Hebei Lulong region from 2009 to 2011. We randomly selected five GARV G9P[8] strains in Hebei Lulong region from 2009 to 2011, amplified the 11 gene fragments of GARVs by RT-PCR, and analyz their full-genome sequences by homology and phylogenetic analysis with DNAStar and MEGA. The nucleotide homology between strains LL11131077 and LL11131083 in 2011 was significantly higher than hat etween them and the other three strains in 2009 and 2010. The G9P[8] GARVs circulating in Hebei Lulong region from 2009 to 2011 elenged to the same genotype as the prevalent G9P[8] GARVs in other parts of the world. However,the two strains in 2011, compared with those in 2009 and 2010, were located in a different sub-branch of the phylogenetic tree and had amino acid mutations at many sites.

关 键 词：轮状病毒 全基因组 G9P［8] 变异 

分 类 号：R373.9[医药卫生—病原生物学]