山东省脑膜炎和脑炎病例中埃可病毒6型的基因特征分析  

Genetic Characterization of Echovirus 6 Isolated from Meningitis and Encephalitis Cases in Shandong Province,China

作　　者：林小娟[1,2] 陶泽新[1,2] 刘桂芳[1,2] 王敏[1,2] 宋立志[1,2] 王素婷[1,2] 纪峰[1,2] 王海岩[1,2] 徐爱强[1,2] 

机构地区：[1]山东省疾病预防控制中心 [2]山东大学预防医学研究院 山东省传染病预防控制重点实验室,济南250014

出　　处：《病毒学报》2014年第2期143-147,共5页Chinese Journal of Virology

基　　金：山东省医药卫生重点项目计划课题(2011QZ013);国家"十二五"科技重大专项"病毒性传染病病原谱流行规律及变异研究"课题(2013ZX10004202)

摘　　要：为分析山东省脑膜炎和脑炎病例中埃可病毒6型（Echovirus6，E6）的基因特征，本研究对山东省2007～2012年间采集的脑膜炎和脑炎病例脑脊液标本进行了病毒分离，阳性分离物采用血清学和分子生物学方法鉴定型别，与国内外其他E6进行同源性分析，并构建VPl完整编码区系统进化树。本研究共分离到6株E6病毒，同源性分析显示：这6株分离株之间核苷酸和氨基酸同源性分别为78．6％～99．8％和95．5％～100．0％，与原型株（D’Amori）核苷酸和氨基酸同源性分别为为76．9％～78．4％和92．3％～95．1％。系统进化树分析显示：山东省E6分离株可分为A、B、C和D4个基因簇，这6株分离株属于A、B、D3个簇。山东省E6分离株之间存在较大的遗传差异，E6在山东的循环存在不同的传播链。To analyze the genetic characteristics of echovirus 6 （E6） isolated from meningitis and encephalitis cases in Shandong Province, China, we collected cerebrospinal fluid samples from meningitis and encephalitis eases in Shandong Province from 2007 to 2012 for virus isolation. Viral RNAs were extracted from positive isolates, and complete VP1 coding regions were amplified by RT-PCR and sequenced. Ho- mology comparison and phylogenetie analysis were performed. Six isolates were identified as E6 by micro- neutralization assay and molecular typing. The homology analysis showed that the six isolates had 78.6 %- 99.8% nucleotide and 95.5%-100.0% amino acid identities with each other, as well as 76.9%-78.4% nueleotide and 92.3%-95.1% amino acid identities with the prototype strain （D＇Amori）. The phylogenetic analysis based on the integrated VP1 sequences indicated that all Shandong E6 isolates could be separated into four clusters, designated as A, 13, C, and D. The six E6 isolates belonged to clusters A, B, and D. Our study reveals high genetic differences between Shandong E6 isolates and suggests different transmission lineages of E6 co-circulated in Shandong Province.

关 键 词：脑膜炎和脑炎病例 埃可病毒6型 系统进化分析 基因 序列同源性 

分 类 号：R373.2[医药卫生—病原生物学]