大豆油分含量稳定性QTL定位  

QTL Analysis of Stability for Oil Content in Soybean

作　　者：沈岩茹[1] 刘春燕[2] 姜振峰[1] 王琳琳[1] 马占洲[1] 杨振[1,3] 辛大伟[1] 蒋洪蔚[2] 胡国华[2,4] 陈庆山[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]黑龙江省农垦科研育种中心,哈尔滨150090 [3]南京林业大学森林资源环境学院,南京210037 [4]国家大豆工程技术研究中心,哈尔滨150050

出　　处：《分子植物育种》2014年第2期254-261,共8页Molecular Plant Breeding

基　　金：黑龙江省教育厅新世纪优秀人才项目(1252-NCET-004);863计划(2012AA101106);国家现代农业产业体系建设专项(CARS-04-02A);黑龙江省自然科学基金重点项目(ZD201213);国家"十二五"科技支撑计划项目(2011BAD35-B06-1)共同资助

摘　　要：以美国半矮杆大豆品种Charleston为母本（早），中国品系东农594为父本（6）杂交，衍生的F2：12-F2：14 。含147个重组自交系，2007～2009年分别在3个环境下种植，测定收获大豆种子油分含量。采用Shukla法计算油分含量的遗传稳定性，将稳定性结果利用复合区间模型和混合线性模型的复合区间作图法进行QTL分析。4个大豆油分稳定性QTL（sqOIL7-1，sqOIL8-1，sqOIL15-1和sqOIL12-1）通过复合区间作图法检测到，他们分别位于连锁群D1a、D1b、J和G上，贡献率分别为5．33％、14．61％、5．83％和5．37％。混合区间作图法检测到4对上位性QTL，贡献率分别为5．65％、11．42％、15．19％和18．32％。研究结果对大豆油分含量重要基因的挖掘及油分相关性状的分子辅助育种提供了重要理论依据。The F2：12-F2：14 RIL population including 147 lines derived from a cross between Charlestonan （An Ame- rican semi-dwarf cultivar） and Dongnong 594 （a Chinese line） were used to identified quantitative trait loci （QTL） associated with oil content. We planted the RIL population from 2007 to 2009 in three different envi- ronments. The seeds were harvested for the measurement of oil content. Shukla A_NOVA method was used to analyze the genetic stability of oil content, and with which the related QTLs were mapped by Composite Interval Mapping （CIM） and Mixed Composite Interval Mapping （MCIM）. Four QTLs （sqOIL7-1, sqOIL8-1, sqOIL15-1, and sqOIL12-1） on Dla, Dlb, J, and G linkage groups were detected for stability of oil content by CIM, accounting for 5.33%, 14.61%, 5.83%, and 5.37% of the general phenotypic variation, respectively. By MCIM, four pair epistaitc QTLs were detected, accounting for 5.65%, 11.42%, 15.19% and 18.32% of the general phenotypic variation, respectively. The results of this study supply an important theoretical foundation to the gene mining and molecular assistant breeding of soybean oil content.

关 键 词：大豆 油分 QTL 稳定性 

分 类 号：S565.1[农业科学—作物学]