物理抗微生物膜与常用抗菌药物对糖尿病足耐药菌株药敏情况的比较研究  

Comparative study on susceptibility of physical antimicrobial film and common antibiotics against drug-resistant strains isolated from diabetic foot

作　　者：梅清华[1] 范翠琼[1] 杨茵[2] 李明友[3] 林茂锐[3] 

机构地区：[1]广东省第二人民医院药学部,广州510317 [2]广东省第二人民医院内分泌科,广州510317 [3]广东省第二人民医院检验科,广州510317

出　　处：《国际医药卫生导报》2014年第7期971-974,共4页International Medicine and Health Guidance News

摘　　要：目的 研究创新物理抗微生物膜对临床糖尿病足耐药菌株的药敏情况.方法 对168例糖尿病足感染患者行病原菌分离培养,以最低抑菌浓度（MIC）稀释法进行药敏试验,比较常用抗菌药物及物理抗微生物膜的耐药情况.结果 分离出的63株菌株按构成比例由多到少依次为：金黄色葡萄球菌、铜绿假单胞菌、产气肠杆菌、阴沟肠杆菌、不动杆菌、表皮葡萄球菌.金黄色葡萄球菌对青霉素、左氧氟沙星、氨苄西林、苯唑西林、哌拉西林、庆大霉素、头孢唑林、头孢他啶等8种抗菌药物耐药率为23.5％～100％;铜绿假单胞菌为23.5％～97.8％;产气肠杆菌为7.5％～75.3％;阴沟肠杆菌为30.5％ ～ 94.6％;不动杆菌为21.6％ ～ 94.6％;表皮葡萄球菌为5.6％～83.8％.以上6种分离菌对物理抗微生物膜耐药率为0.结论 物理抗微生物膜具有广谱抗菌,对各种细菌敏感率高的特点,为临床治疗糖尿病足提供了有效,而且可避免耐药的物理学抗感染新方法.Objective To study susceptibility of innovative physical method against resistant strains isolated from clinical diabetic foot.Methods Pathogens from 168 cases of patients with diabetic foot infections were isolated and cultured,minimum inhibitory concentration （MIC） dilution method was adopted for susceptibility test to compare drug resistance of Physical Antimicrobial Film and common antibiotics.Results 63 strains were cultured and isolated from affected parts of diabetic foot patients.According to ratio,the strains from the maximum to minimum were：Staphylococcus aureus,Pseudomonas aeruginosa,Enterobacter aerogenes,Enterobacter cloacae,Acinetobacter,Staphylococcus epidermidis.The drug-resistant rates of Staphylococcus aureus to penicillin,levofloxacin,ampicillin,oxacillin,piperacillin,gentamicin,cefazolin,ceftazidime were 23.5％ to 100％; those of Pseudomonas aeruginosa to eight antibiotics were 23.5％ to 97.8％;those of Enterobacter aerogenes to eight antibiotics were 7.5％ to 75.3％; those of Enterobacter cloacae to eight antibiotics were 30.5％ to 94.6％; those of Acinetobacter to eight antibiotics were 21.6％ to 94.6％;those of Staphylococcus epidermidis to eight antibiotics were 5.6％ to 83.8％.The drug-resistant rates of the above six isolated strains to Physical Antimicrobial Film JUC Spray Dressing were 0.Conclusion Physical Antimicrobial Film has the characteristics of broad-spectrum antimicrobe,with high sensitive rates to a variety of bacteria.It provides a new effective anti-infective physics （not chemical or biological） method and can avoid drug resistance for the clinical treatment of diabetic foot.

关 键 词：耐药菌 糖尿病足 药敏 物理抗微生物膜 抗菌药物 

分 类 号：R446.5[医药卫生—诊断学]