头孢噻肟钠治疗小儿化脓性脑膜炎作用机制研究  

The Role of Cefotaxime Sodium on Clinical Pediatric Purulent Meningitis

作　　者：王蕊艳[1] 谢基华[1] 

机构地区：[1]江西省儿童医院神经内儿科,南昌330006

出　　处：《中国医药导刊》2013年第12期2061-2063,2065,共4页Chinese Journal of Medicinal Guide

摘　　要：目的：探讨头孢噻肟钠（凯福隆）治疗小儿化脓性脑膜炎作用机制研究。方法：自2009年3月~2011年9月我院接诊小儿化脓性脑膜炎患儿79例,均经临床表现、脑脊液检查、颅脑CT以及血常规检查等方法确诊。根据随机数字表法将79例患儿随机分为两组,即头孢唑肟治疗组、凯福隆治疗组,另选取健康同龄儿童30例作为健康对照组。分别通过ELISA、荧光定量PCR法以及蛋白免疫印迹（Westerblot）法,在药物治疗前及治疗后对细胞炎性因子中的肿瘤坏死因子α（TNF-α）、白介素6（IL-6）、白介素1β（IL-1β）以及基质金属蛋白-9（MMP9）的蛋白含量或者mRNA含量进行测定。分析相关药物的具体治疗机制。结果：ELISA法结果显示,相比于头孢唑肟组,凯福隆组在治疗后的炎性细胞因子的蛋白含量显著降低（P〈0.01）,同时通过荧光定量PCR法结果也保持一致,具有显著的统计学差异（P〈0.01）。而通过蛋白免疫印迹法检测MMP-9蛋白含量发现,头孢唑肟组治疗后未有明显降低,而凯福隆组治疗后,MMP-9蛋白含量显著降低,具有显著的统计学差异（P〈0.01）。结论：结合MMP-9在血脑屏障中的影响,凯福隆药物通过显著降低MMP-9水平从而改善患儿脑膜炎症状,为今后研究小儿细菌性及化脓性脑膜炎发病机制提供了一定的理论基础及实验基础。Objective：To explore the role of Cefotaxime Sodium on pediatric purulent meningitis. Methods：From 2009 March to 2011 September in our hospital with admissions of children with bacterial and purulent meningitis in 79 cases, which is through the clinical presentation, examination of cerebrospinal fluid, brain CT and blood routine examination and other methods confirmed. According to the random number table：79 patients were randomly divided into two groups, namely of Ceftizoxime in the treatment group,Cefotaxime Sodium group, in addition the other children of the same age in 30 cases of healthy as a healthy control group. Through ELISA, fluorescence quantitative PCR assay and Western blot （Westerblot ）method, before and after medication take a few therapy on inflammatory cytokine production in tumor necrosis factorα（TNF-α）,interleukin 6 （IL-6）,interleukin 1β（ IL-1β） and matrix metalloproteinase-9 （MMP-9）.Protein content and mRNA content determination. Analysis of related drugs to specific treatment mechanism. Results：The ELISA method results show compared to Ceftizoxime group, Cefotaxime Sodium group after treatment on inflammatory cytokine protein content decreased significantly （P〈0.01）,at the same time by fluorescence quantitative PCR assay results are consistent, with significant statistical difference （P〈0.01）.Through protein immunoblot assay for detection of MMP-9 protein content discovery, Ceftizoxime group after treatment did not significantly decreased, and Cefotaxime Sodium group after treatment, the MMP-9 protein content was lower, with significant statistical difference （P〈0.01）. Conclusion：According to the role of MMP-9 in the blood brain barrier, Kefalo drugs with MMP-9 level significantly reduced so as to improve the patients symptoms of meningitis, this thesis of the paper on pediatric bacterial and purulent meningitis pathogenesis can be as the theoretical basis and experimental base.

关 键 词：凯福隆 小儿化脓性脑膜炎 

分 类 号：R725.1[医药卫生—儿科]