茚虫威对玫瑰三节叶蜂叶绿素和类胡萝卜素含量的影响  

Effects of inodoxacarb on chlorophyll and carotenoid contents of Arge geei

作　　者：李新扬[1] 徐元元[1] 公玲玲 周科[1] 井维霞[1] 王涛 李慧婵 曲爱军[1] 

机构地区：[1]山东农业大学植物保护学院,山东泰安271018 [2]山东省聊城市林业局,山东聊城252000 [3]山东安丘实验中学,山东潍坊262100

出　　处：《生物安全学报》2013年第4期261-264,共4页Journal of biosafety

基　　金：山东农业大学SRT资助项目(2013)

摘　　要：【背景】研究昆虫体色不仅在昆虫系统学中,而且在生物学、生态学和害虫防治等中都具有重要意义。有些取食植物叶片的幼虫体色表现为绿色,该绿色是由类胡萝卜素与蓝色四吡咯色素结合所形成,叶绿素又属于四吡咯化合物。但目前尚未见昆虫体内含叶绿素的报道。【方法】在田间套笼饲养玫瑰三节叶蜂幼虫,饲养至5和6龄时,借鉴植物叶绿素测定方法,分别测定其叶绿素含量,并在6龄时用杀虫剂茚虫威处理,观测对其叶绿素含量的影响。【结果】5和6龄玫瑰三节叶蜂幼虫均含有叶绿素,且5龄幼虫含量高于6龄幼虫。其中,5龄幼虫叶绿素a含量为1.6780 mg·g-1,叶绿素b为0.6847 mg·g-1,类胡萝卜素为0.4797 mg·g-1;6龄幼虫叶绿素a含量为1.2726 mg·g-1,叶绿素b为0.5187 mg·g-1,类胡萝卜素为0.4863 mg·g-1。剔除幼虫消化道后,5龄幼虫叶绿素a含量为0.4008 mg·g-1,叶绿素b为0.2618 mg·g-1,类胡萝卜素为0.2444 mg·g-1;6龄幼虫叶绿素a含量为0.4299 mg·g-1,叶绿素b为0.3826 mg·g-1,类胡萝卜素为0.2859 mg·g-1。用茚虫威处理后,6龄幼虫体内叶绿素和类胡萝卜素含量(含消化道)显著下降,其中,叶绿素a下降约48%,叶绿素b下降约34%,类胡萝卜素下降约37%;而对于剔除消化系统的6龄幼虫,茚虫威处理后,其叶绿素和类胡萝卜素含量下降幅度较小。【结论与意义】玫瑰三节叶蜂幼虫含有叶绿素和类胡萝卜素,杀虫剂能降低其叶绿素和类胡萝卜素含量,且其体色可能与叶绿素有着密切的关系。[ Background ] The study of insect color is significant not only in insect morphology, but also for its ecology and control management. The green color of insects is usually formed by the carotenoid and pigment porphyrin. [ Method ] To explore the rela- tionships between insect color and plant chlorophyll, the contents of chlorophyll and carotenoid of Arge geei Rohwer which were raised using cage in the field were tested. [ Result]The results showed that both 5th instar and 6th instars larvae had chlorophyll. The chlorophyll contents of 5th instar larvae were higher than 6th instar larvae. For fifth instars, the contents of chlorophyll a and chlorophyll b were 1.6780 mg g- and 0.6847 mg g-i respectively, the content of carotenoids was 0.4797 mg g-i; for sixth in- stars, the contents of chlorophyll a and chlorophyll b were 1.2726 mg g-I and 0.5187 mg g i, the content of carotenoids was 0.4863 mg g-J. After removed the digestive system, the contents of chlorophyll a, chlorophyll b of fifth and sixth instars were respective- ly 0.4008 mg g -, 0.2618 mg g - l and 0.4299 mg g - J , O. 3826 mg g - 1 , the contents of carotenoids were 0.2444 mg g - i and 0.2859 mg g-J. After treating with indoxacarb, the chlorophyll contents of sixth instar larvae with digestive system significantly declined. Chlorophyll a dropped by about 48%, chlorophyll b by about 34%, and carotenoids by about 37%. While the chlorophyllcontents of sixth instar larvae without digestive system decreased slightly. [ Conclusion and significance ] The larvae of A. geei contain chlorophyll and carotenoid, insecticides can reduce the contents of them. The color of A. geei and the chlorophyll have closely rela- tionship.

关 键 词：玫瑰三节叶蜂 叶绿素 类胡萝卜素 茚虫威 

分 类 号：S482.3[农业科学—农药学]