重组人白细胞介素-6包含体溶解工艺研究  

A Study for Solubilization of rhIL 6 Inclusion Body Produced from E.Coli

作　　者：吴蕾[1] 张瑛[1] 甘一如[1] 孙江莉 张浩森 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《化学工业与工程》2000年第6期321-325,共5页Chemical Industry and Engineering

基　　金：国家“九五”攻关项目(96-C02-01-04)

摘　　要：本文系统研究了影响大肠杆菌表达的重组人白细胞介素 6 (rhIL 6 )包含体溶解的因素 ,确定了rhIL 6包含体溶解的最佳工艺条件 ,即在 pH为 8 4的 5 0mmol/LTris HCL缓冲体系中 ,变性剂尿素和还原剂 β 基乙醇 (β ME)的浓度分别为 8mol/L和 5 0mol/L ,包含体含量为 1 0mg/mL ,在 4℃下溶解 1h。包含体溶解后蛋白含量可达到 1 3mg/mL左右 ,目标蛋白的溶解率达到 98% ,纯度为 5 0The technological condition for solubilization of the recombinant human Interleuk in\|6(rhIL 6) inclusion body produced from E.coli was studied. The optimal solubilization condition was obtained:the inclusion body was solubilized at 4℃ in 50mmol/L Tris\|HCL(pH=8.4),8mol/L urea and 50 mM β ME at a concentration of 10mg/mL for about 1h. Then nearly all the target protein was solubilized and the solubilized protein concentration was solubilized and the solubilized protein concentration was about 1.3 mg/mL and the purity was up to 50%.

关 键 词：重组人白细胞介素-6 包含体 溶解 

分 类 号：TQ464.9[化学工程—制药化工]