克拉维酸产生菌的菌种选育  

Breeding of Clavulanic Acid-producing Strain

作　　者：俞岩青 田敏[1] 林明[1] 蒋用[1] 王昆蓉[1] 

机构地区：[1]四川抗菌素工业研究所,四川成都610052

出　　处：《中国医药工业杂志》2014年第4期322-324,共3页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2011ZX09203-001-19)

摘　　要：采用紫外线对克拉维酸产生菌SIIA 0487原生质体进行诱变,并以苏氨酸耐受为筛选模型,从含8%苏氨酸的再生培养基上筛选到一株高产菌株SIIA 0220,在摇瓶试验中,该菌株对苏氨酸的耐受浓度由6%提高到10%,克拉维酸产量较出发菌株提高约36%,且遗传稳定性良好。The protoplast of clavulanic acid-producing strain SIIA 0487 was mutagenized by ultraviolet radiation. A threonine resistance mutant named as SIIA 0220 was obtained on the regeneration medium containing 8 % threonine. The tolerance level of threonine increased from 6 % to 10%. The yield of clavulanic acid increased by 36% in shake flask fermentation compared with the parent strain. Its genetic stability was good.

关 键 词：克拉维酸 原生质体诱变 苏氨酸 筛选模型 

分 类 号：Q933[生物学—微生物学]