广东紫珠药材的UFLC指纹图谱  

Fingerprint Chromatography of Callicarpa kwangtungensis Chun by UFLC

作　　者：李丽[1] 杨义芳[1] 胡晓[1] 萧伟[2] 王振中[2] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院 创新药物与制药工艺国家重点实验室,上海200040 [2]江苏康缘药业股份有限公司,江苏连云港222001

出　　处：《中国医药工业杂志》2014年第4期330-333,共4页Chinese Journal of Pharmaceuticals

基　　金：国家“重大新药创制”科技重大专项(2009ZX09301-007);2013年上海市中药领域科技支撑项目(13401900702);2013年上海市青年科技基金项目(132R1461700)

摘　　要：建立了广东紫珠药材的超快速液相色谱指纹图谱,并对其进行化学模式识别。采用Ultimate XB-C18色谱柱,以乙腈︰0.5%磷酸为流动相,梯度洗脱,检测波长332 nm。结果显示17个共有峰,其DAD紫外光谱信号主要分为两类;所建立的指纹图谱能够有效区分正品与疑似品,有助于广东紫珠药材的质量控制。An UFLC method was used for the establishment of fingerprint chromatography of Callicarpa kwangtungensis Chun and the fingerprint chromatography was performed with chemical recognition mode. An Ultimate XB-C18 column was used with the mobile phase ofacetonitrile ： 0.5 % phosphoric acid by gradient elution at the detection wavelength of 332 nm. Seventeen common peaks were determined in UFLC fingerprint and were divided into two categories according to the DAD spectrum analysis. The UFLC fingerprint chromatography could distinguish Callicarpa kwangtungensis Chun from the suspected varieties and was effective for its quality control.

关 键 词：广东紫珠 指纹图谱 超快速液相色谱 

分 类 号：O657.72[理学—分析化学] R93[理学—化学]