补肾益寿胶囊水提液的精制工艺  

Refi nement of Water Extract of Bushen Yishou Capsules

作　　者：王欣[1] 罗维早[1] 王爱平[1] 彭涛[2] 覃瑶[2] 

机构地区：[1]重庆市中药研究院中药药物化学研究所,重庆400065 [2]太极集团有限公司,重庆401147

出　　处：《中国医药工业杂志》2014年第4期334-338,共5页Chinese Journal of Pharmaceuticals

基　　金：国家科技部"十一五""重大新药创制"科技重大专项(2009ZX09313-037);重庆市科研院所创新能力建设计划项目(cstc2012pt-kyys10001;cstc2012pt-kyys10004)

摘　　要：考察了Seplite LX-66型大孔吸附树脂对补肾益寿胶囊5味药材水提液的精制工艺,筛选最佳条件并探讨大孔吸附树脂再生条件及老化情况。建立了补肾益寿胶囊5味药材水提液的HPLC指纹图谱,以选定的10个共有色谱峰为指标,结合70%乙醇洗脱液干膏收率评价Seplite LX-66型大孔吸附树脂上样量、洗脱条件和树脂再生条件及老化情况。精制工艺的最佳条件为:上样温度60℃,水提液上样量为2倍大孔吸附树脂干重,水和70%乙醇洗脱2.7和2.0倍柱体积。以该工艺重复操作17批次,大孔吸附树脂未老化。The refining process of water extract of Bushen Yishou capsules by Seplite LX-66 macroporous resin was studied. The optimal conditions for the refining process were selected and the renewing and aging conditions of macroporous resin were obtained. With the help of HPLC fingerprint chromatogram of the water extract of Bushen Yishou capsules, ten common peaks and dry extract yield by 70 % ethanol eluting fraction were chosen as the indexes for evaluating the loading amount, elution condition and the renewing and aging conditions of Seplite LX-66 macroporous resin. The results revealed that the optimal conditions for refining process were as follows： sample temperature of 60 ℃, loading amount equal to 2 times of the dry weight of macroporous resin, water and 70 % ethanol eluting volume of 2.7 and 2.0 times of the macroporous resin, respectively. The macroporous resin wasn＇t aged after repeated for 17 batches.

关 键 词：补肾益寿胶囊 精制 大孔吸附树脂 

分 类 号：R93[医药卫生—生药学]