感染科医务人员甲型H1N1流感血清抗体消长的比较分析  

Comparative analysis of influenza A( H1N1) sero-antibody levels and vaccination effectiveness in medical staff of infection department

作　　者：李建芳[1] 蒋素文[1] 胡爱荣[1] 宣王益 郑南红[1] 高国生[1] 

机构地区：[1]宁波市第二医院感染科,浙江宁波315010 [2]宁波市镇海区中医医院内科,浙江宁波315010

出　　处：《中国卫生检验杂志》2014年第6期834-837,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省省市共建医药卫生科技计划项目(2010S-SA003);浙江省医坛新秀培养对象项目(2013-245);宁波市优秀中青年卫生技术人才资助项目(2011-145);宁波市领军和拔尖人才培养工程资助项目(2012-131)

摘　　要：目的分析感染科医务人员甲型H1N1流感血清抗体消长变化,为制定免疫策略提供依据。方法 339名自愿接种甲型H1N1流感疫苗的感染科医务人员在接种后6个月、1年、2年采集血清,63名不愿意接种的感染科医务人员在相同时间采血,分别检测其IgM及IgG抗体。结果接种组与未接种组比较,接种后6个月、1年及2年时,其IgM抗体和IgG抗体水平和阳性率差异均有统计学意义(P<0.05);接种组IgM抗体水平在接种后6个月最高(A值1.00±0.22),而IgG抗体在接种后1年最高(A值1.02±0.16),其IgM和IgG抗体水平在各个时间段之间的两两比较差异均有统计学意义(P<0.001);Logistic回归分析在接种后的6个月、1年和2年,性别和年龄两个变量差异均无统计学意义(P>0.05)。结论甲型H1N1流感疫苗的免疫原性较理想,尤其是感染科医务人员,目前易感人群又在不断增加,有必要将其纳入常规接种及加强免疫。Objective To analyze the influenza A （ H1 N1 ） sero - antibody levels and vaccination effectiveness in medical staff of infection department, and to provide evidence for making immunization strategy. Methods Serum specimens were collected from 339 medical staffs with voluntary vaccination at the 6th month, 1 st year and 2nd year after vaccination. And serum speci- mens were collected from 63 non - vaccinated medical staffs of infection department at the same time for comparison. Antibody titres （IgM and IgG） were measured with enzyme linked - immunosorbent assay （ELISA） in these serum specimens. Results The titres and positive rates of IgM and IgG in vaccine recipients were higher than those in non - vaccine recipients at the 6th month, 1st year and 2nd year after vaccination （P 〈0.05）. The titres of IgM in vaccine recipients were at the highest level （A value 1.00 ± 0.22） at the 6th month after vaccination and then gradually decreased, while the titres of IgG in vaccine recipients were at the highest level （A value 1.02 ±0.16） at 1st year after vaccination. The pairwise comparison of IgM and IgG levels in vaccine recipients showed significant statistical differences at the 6th month, Ist year and 2nd year after vaccina- tion （P 〈0.001 ）. The levels of IgM and IgG were not correlated with gender and age by logistic regression analysis. Conclu- sion The immunogenicity of influenza A （H1 N1 ） vaccines is satisfying in China, especially among medical staff of infection department. Due to the increasing trend of susceptible population, it is necessary to take influenza A（ H1 N1 ） vaccination from emergency vaccination to routine immunization and strengthened immunization.

关 键 词：流感病毒A型 H1N1亚型 疫苗 免疫原性 

分 类 号：R186[医药卫生—流行病学]