细菌视紫红质膜的光电荷转移动力学过程的研究  

The Kinetics of Photo-Charge Transfering in Bacteriorhodopsin

作　　者：黄玉华[1] 赵有源[1] 欧阳晓平[1] 龚勤敢[1] 杨群[1] 陈凌冰[1] 李富铭[1] 张忠宾 刘坚[3] 丁建东[3] 李庆国[4] 

机构地区：[1]复旦大学物理系.光科学及工程系三束材料改性国家重点实验室,上海200433 [2]西北工业大学声学工程研究所,西安710072 [3]复旦大学高分子科学系,上海200433 [4]复旦大学生物与生物物理学系,上海200433

出　　处：《光学学报》2001年第1期106-110,共5页Acta Optica Sinica

基　　金：国家自然科学基金!(198340 30和 6 98880 0 1)资助项目

摘　　要：实验测量了细菌视紫红质 (bR)分别经波长为 10 6 4nm、5 32nm和 35 5nm ,脉冲宽度为 10ns的三种不同的脉冲激光照射后产生的不同的光电压信号 ,着重用 5 32nm脉冲光激发样品 ,从变化的测量回路的阻抗所得到的不同光电信号情况 ,研究并分析了细菌视紫红质的光电荷的产生和转移的微观机制。结果表明 ,其正负脉冲光电压信号是正负两种电荷移动的结果 ,而正电荷的产生比负电荷的产生需要克服更高的能量 ,同时 ,正负电荷既具有各自的转移特性又相互影响 ,如正电荷移动速度慢 ,负电荷移动速度快 ,负电荷对正电荷有牵引作用等 ,这些特性与电极和样品之间形成的界面效应有关 。The different photoelectric signals in bacteriorhordopsin are experimentally measured when it is excited by 10 ns pulsee lasers at 1064 nm, 532 nm and 355 nm. 532 nm pulse laser is used mostly as pumping light to measure the photoelectric signals affected by the impedance of the measuring circuit. The mechanism that bacteriorhodopsin generates photo charges and the photo charges transfer in it was studied and analyzed. The results show that the positive and negative photoelectric signals are produced by the shift of the positive and negative charges respectively, and more energy is needed to produce positive charges than negative ones. At the same time, the positive and negative charges not only have their own transfering characteristics, but also affect each other, such as, positive charges move slowly, and negative ones fast, moreover, the negative can pull the positive to move forward, which, together with the interface between the sample and electrodes, cause the magnitude and width of the pulse photo voltage having special dependence different from other photo electronic devices on the linked resistance.

关 键 词：细菌视紫红质 光电压信号 电荷转移 动力学 生物膜 

分 类 号：Q735[生物学—分子生物学]