不同电针刺激对SAMP8小鼠学习记忆能力及大脑皮层APP、ApoE mRNA表达的影响  

Effect of Electroacupuncture Intervention on Learning- memory Ability,APP and ApoE mRNA Expression in Cerebral Cortex of SAMP8

作　　者：许安萍[1] 唐银杉[1] 陈万顺[1] 莫雨平[1] 姚海江[1] 赛因朝克图 李志刚[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《针灸临床杂志》2014年第2期62-65,共4页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金;编号:81273825

摘　　要：目的:探讨脉冲电针和音乐电针治疗阿尔茨海默病(AD)的可能作用机制,并比较两种电针的疗效差异。方法:将SAMP8小鼠随机分为模型组、脉冲电针组和音乐电针组,SAMR1小鼠作为正常组。两电针组均给予电针百会穴、印堂穴20 min后,点刺水沟穴。治疗结束后,以Morris水迷宫测定小鼠学习记忆能力,实时荧光定量PCR法测定小鼠大脑皮层中APP、ApoE mRNA表达量。结果:与模型组比较,音乐电针组、脉冲电针组逃避潜伏期分别在第4天、第5天明显下降(P<0.05),目标象限游泳时间均增加(P<0.05);脉冲电针组、音乐电针组大脑皮层APP mRNA表达量均显著下降(P<0.05)。结论:脉冲电针和音乐电针均可改善SAPM8小鼠学习记忆能力;脉冲电针和音乐电针可能通过下调APP mRNA表达发挥抗痴呆作用。Objective：To observe the influence of music electroacupuncture （EA） and pulse EA on learning memory ability and APP and ApoE mRNA expression in cerebral cortex of SAMPS, so as to explore their mech- anisms underlying relief of Alzheimer＇ s Disease （AD）. Methods ： SAMP8 mice were randomly divided into mod- el, pulse EA and music EA groups. SAMR1 mice were used as the control group. EA was applied to ＂Baihui＂ （ GV20 ） and ＂Yintang＂ （ EX - HN3 ）, and then swift pricked ＂Shuigou＂ （ GV26 ）. The learning memory ability of mice was detected by using Morris Water Maze. APP and ApoE mRNA expression level in cerebral cortex were assayed by fluorescent quantitative real - time PCR. Results： Compared with the model group, the escapel- atency of music EA was decreased considerably on the fourth day （ P 〈 0.05 ） , but pulse EA was on the fifth day, as well as the level of APP mRNA expression was down - regulated obviously in pulse EA and music EA. Conclusion：Both pulse EA and music EA can effectively improve learning memory ability of SAMP8 mice which may be closely associated with their effects on down -regulating APP mRNA expression in cerebral cor- tex.

关 键 词：阿尔茨海默病 脉冲电针 音乐电针 Β-淀粉样前体蛋白 载脂蛋白E 

分 类 号：R245.97[医药卫生—针灸推拿学]