家蚕生物钟蛋白TIME-EA4的进化踪迹分析  

The Evolutionary Trace Analysis of Biological Clock Protein TIME-EA4 in the Silkworm Bombyx mori

作　　者：王文栋[1] 徐世清[2] 

机构地区：[1]河北北方学院医学检验学院,河北张家口075000 [2]苏州大学医学部,江苏苏州215123

出　　处：《四川动物》2014年第2期184-189,共6页Sichuan Journal of Zoology

基　　金：国家自然科学基金项目"家蚕滞育生物钟蛋白质EA4的分子作用机理研究"(30371086);国家自然科学基金项目"基于ea4与sod基因互作的滞育生物钟分子调控机制研究"(30771632)

摘　　要：家蚕是卵滞育性鳞翅目模式昆虫,其滞育卵的活化与生物钟蛋白TIME-EA4密切相关。TIME-EA4具备稳定的Cu/Zn SOD活性和瞬时的ATP酶活性。目前,国内外关于家蚕TIME-EA4的研究主要集中在结构和功能上,其分子进化机制研究尚未开展。本文利用生物信息学方法对TIME-EA4进行了进化踪迹分析,结果显示TIME-EA4的重要氨基酸残基(coverage<25%)的91.2%都用来维持与Cu/Zn SOD的序列一致性,但TIME-EA4与Cu/Zn SOD的Cu/Zn离子结合位点在氨基酸残基组成、极性、绑定原子方面都存在差异。Bombyx mori is the model insect of Lepioloptera with diapause during the egg stage, and the activation of diapause eggs is closely related to the biological clock protein TIME-EA4. TIME-EA4 has stable Cu/Zn SOD activity and one-time transitory ATPase activity. Numerous studies have focused on the structural and functional characteristics of TIME-EA4. The molecular evolution of this protein has received little attention. In this study, bioinformatics methods were used to analyze the evolutionary trace of TIME-EA4. The results showed that 91.2% of the important amino acid residues in TIME-EA4 were consistent with Cu/Zn SOD. However, there were some differences such as composition, amino acid residue polarity and bonded atoms in Cu/Zn ion binding sites were observed between these proteins.

关 键 词：家蚕 生物钟 生物信息学 分子进化 进化踪迹 

分 类 号：S881.24[农业科学—特种经济动物饲养]